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N-Hydroxysuccinimidyl-4-azidobenzoate (CAS 53053-08-0)

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Application:
N-Hydroxysuccinimidyl-4-azidobenzoate is an aryl azide-based, amine- and photoreactive crosslinker
CAS Number:
53053-08-0
Purity:
>97%
Molecular Weight:
260.21
Molecular Formula:
C11H8N4O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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N-Hydroxysuccinimidyl-4-azidobenzoate (NHS-4-azidobenzoate) is a chemical compound used in bioconjugation chemistry to introduce azide groups into biomolecules. This compound combines an N-hydroxysuccinimide (NHS) ester with a 4-azidobenzoate moiety, making it a versatile reagent for modifying proteins, nucleic acids, and other biological targets with azides. The NHS ester is a reactive group that forms stable amide bonds with primary amines, which are found in the side chains of lysine residues in proteins or the amino termini of peptides and proteins. The reaction between the NHS ester and an amine is efficient and occurs under mild conditions, making NHS esters popular for bioconjugation applications.


N-Hydroxysuccinimidyl-4-azidobenzoate (CAS 53053-08-0) References

  1. Ca2+-calmodulin inhibits Ca2+ release mediated by type-1, -2 and -3 inositol trisphosphate receptors.  |  Adkins, CE., et al. 2000. Biochem J. 345 Pt 2: 357-63. PMID: 10620513
  2. Solubilization of functional receptors for parathyroid hormone and parathyroid hormone-related peptide from clonal rat osteosarcoma cells, ROS17/2.8.  |  Uneno, S., et al. 1992. Calcif Tissue Int. 51: 382-6. PMID: 1333876
  3. Affinity purification of human granulocyte macrophage colony-stimulating factor receptor alpha-chain. Demonstration of binding by photoaffinity labeling.  |  Chiba, S., et al. 1990. J Biol Chem. 265: 19777-81. PMID: 2147182
  4. Solubilization and characterization of active neuropeptide Y receptors from rabbit kidney.  |  Gimpl, G., et al. 1990. J Biol Chem. 265: 18142-7. PMID: 2170404
  5. Identification of a crotoxin-binding protein in membranes from guinea pig brain by photoaffinity labeling.  |  Hseu, MJ., et al. 1990. J Bioenerg Biomembr. 22: 39-50. PMID: 2341382
  6. Biochemical properties of brain somatostatin receptors.  |  Thermos, K., et al. 1989. Neuroscience. 31: 131-41. PMID: 2570375
  7. Photoaffinity labelling of the vasoactive-intestinal-peptide-binding site on intact human colonic adenocarcinoma cell line HT29-D4. Synthesis and use of photosensitive vasoactive-intestinal-peptide derivatives.  |  Martin, JM., et al. 1988. Biochem J. 250: 679-85. PMID: 2839143
  8. Pertussis toxin modifies the characteristics of both the inhibitory GTP binding proteins and the somatostatin receptor in anterior pituitary tumor cells.  |  Mahy, N., et al. 1988. J Pharmacol Exp Ther. 246: 779-85. PMID: 2900331
  9. Molecular characteristics of nerve growth factor receptors on PC12 cells.  |  Hosang, M. and Shooter, EM. 1985. J Biol Chem. 260: 655-62. PMID: 2981225
  10. Cross-linking of a polyomavirus attachment protein to its mouse kidney cell receptor.  |  Griffith, GR. and Consigli, RA. 1986. J Virol. 58: 773-81. PMID: 3009886
  11. N,N'-Bis(4-azidobenzoyl)cystine--a cleavable photoaffinity reagent.  |  Ultee, ME. and Basch, RS. 1985. Anal Biochem. 149: 331-8. PMID: 4073491
  12. A competitive enzyme-linked immunoassay for domoic acid determination in human body fluids.  |  Smith, DS. and Kitts, DD. 1994. Food Chem Toxicol. 32: 1147-54. PMID: 7813986
  13. Cross-linking of Escherichia coli RNA polymerase subunits: identification of beta' as the binding site of omega.  |  Gentry, DR. and Burgess, RR. 1993. Biochemistry. 32: 11224-7. PMID: 8218187
  14. Photoaffinity labeling of the ATP transporter of rat liver microsomes.  |  Kim, SH., et al. 1997. Mol Cells. 7: 582-8. PMID: 9387142

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

N-Hydroxysuccinimidyl-4-azidobenzoate, 100 mg

sc-263835
100 mg
$138.00

What color is this compound?

Asked by: katsoc97
Thank you for your question. Our most recent lot was an off-white solid.
Answered by: Technical Support
Date published: 2019-01-26
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Rated 5 out of 5 by from KimKim, SH. et al. (PubMed 9387142) synthesized a photoreactive azido derivative of ATP, 3 (2')-O-(p-azidobenzoyl)-ATP (AB-ATP), by the reaction of ATP with N-hydroxysuccinimidyl-4-azidobenzoate (NHS-AB) to photolabel the ATP transporter of rat liver microsomes. -SCBT Publication Review
Date published: 2015-06-17
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N-Hydroxysuccinimidyl-4-azidobenzoate is rated 5.0 out of 5 by 1.
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