MYL6B Antibody (YY-06): sc-100951

MYL6B Antibody (YY-06) is a mouse monoclonal IgG2b kappa light chain antibody that detects MYL6B protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-MYL6B antibody (YY-06) is available as a non-conjugated format. MYL6B, or Myosin light chain 6B, plays a crucial role in regulating contractile activity in smooth muscle and non-muscle myosin complexes. MYL6B is primarily located in the cytoplasm, where MYL6B forms a hexameric structure consisting of four light chains and two heavy chains, which is essential for function in muscle contraction. MYL6B presence in smooth muscle cells is particularly important as MYL6B facilitates interaction with myosin heavy chains, enabling force generation and movement in response to calcium signaling. Notably, while MYL6B contains three EF-hand domains typically associated with calcium binding, MYL6B does not bind calcium during contraction, highlighting a unique regulatory role. MYL6B is expressed in various tissues, with highest levels found in neurons and smooth muscle, underscoring significance in both muscle function and cellular signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MYL6B Antibody (YY-06) References:

1. Expression of the alpha 6A integrin splice variant in developing mouse embryonic stem cell aggregates and correlation with cardiac muscle differentiation.  |  Thorsteinsdóttir, S., et al. 1999. Differentiation. 64: 173-84. PMID: 10234814
2. Purification and characterization of smooth muscle myosin-associated phosphatase from chicken gizzards.  |  Mitsui, T., et al. 1992. J Biol Chem. 267: 16727-35. PMID: 1322916
3. Increase of vinblastine accumulation by inhibitors of calmodulin-dependent cell functions in rat ascites hepatoma AH66 cells.  |  Wakusawa, S., et al. 1992. Anticancer Res. 12: 2021-4. PMID: 1363514
4. Myosin light chain isoform expression among single mammalian skeletal muscle fibers: species variations.  |  Bicer, S. and Reiser, PJ. 2004. J Muscle Res Cell Motil. 25: 623-33. PMID: 15750847
5. A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles.  |  Takeya, K., et al. 2008. Am J Physiol Renal Physiol. 294: F1487-92. PMID: 18400874
6. MYL6B, a myosin light chain, promotes MDM2-mediated p53 degradation and drives HCC development.  |  Xie, X., et al. 2018. J Exp Clin Cancer Res. 37: 28. PMID: 29439719
7. Purification and properties of troponin T kinase from rabbit skeletal muscle.  |  Kumon, A. and Villar-Palasi, C. 1979. Biochim Biophys Acta. 566: 305-20. PMID: 33714
8. MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process.  |  Li, JL., et al. 2020. Open Life Sci. 15: 522-531. PMID: 33817240
9. Erratum to 'MYL6B drives the capabilities of proliferation, invasion, and migration in rectal adenocarcinoma through the EMT process'.  |  Wang, ZQ., et al. 2020. Open Life Sci. 15: 797. PMID: 33818581
10. Study on the expression and prognostic relationship of MYL6B in liver cancer based on bioinformatics.  |  Lv, HB., et al. 2024. World J Clin Oncol. 15: 1188-1197. PMID: 39351463
11. Purification and characterization of rabbit liver calmodulin-dependent glycogen synthase kinase.  |  Payne, ME., et al. 1983. J Biol Chem. 258: 2376-82. PMID: 6296143
12. Partial purification and characterization of myosin light chain kinase from bovine thyroid gland.  |  Tawata, M., et al. 1983. Endocrinology. 112: 701-6. PMID: 6687357