Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mts1 Double Nickase Plasmid (h): sc-401774-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mts1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mts1 Double Nickase Plasmid (h) and Mts1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting S100A4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mts1 Antibody (A-7): sc-377059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mts1 Double Nickase Plasmid (h) | sc-401774-NIC | 20 µg | $410.00 | |||
Mts1 Double Nickase Plasmid (h2) | sc-401774-NIC-2 | 20 µg | $410.00 |
S100A4 encodes the calcium-binding protein Mts1, a member of the S100 family that acts as a cytosolic and extracellular regulator of cell motility and cytoskeletal dynamics. Mts1 interacts with targets such as nonmuscle myosin II and modulates processes including actin remodeling, focal adhesion turnover, and epithelial–mesenchymal transition-like programs. Through calcium-dependent signaling and cross-talk with pathways that govern migration and invasion, S100A4 is frequently studied in the context of tumor progression, fibrosis, and inflammatory microenvironments. Dysregulated S100A4 expression is also associated with changes in stromal activation and immune cell trafficking, making it a useful node for dissecting metastasis-associated signaling networks.
Mts1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the S100A4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within S100A4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt S100A4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of S100A4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.