mouse anti-rabbit IgG-CFL 488 mouse monoclonal secondary antibody conjugated to CruzFluor™ 488 (CFL 488), for WB (RGB), IF, IHC(P) and FCM

mouse anti-rabbit IgG-CFL 488: sc-516248

mouse anti-rabbit IgG-CFL 488 Secondary Antibody is rated 4.6 out of 5 by 11.
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Datasheets

    • mouse monoclonal secondary antibody conjugated to CruzFluor™ 488 (CFL 488)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 488 nm, Emission of 514 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
    • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
    • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-CFL 488 (sc-516248), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-CFL 488 secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 5 out of 5 by from IF-C 1:200 Since I started using this secondary antibody, I was not bothered by nonspecific reactions.
Date published: 2019-07-05
Rated 5 out of 5 by from Strong signal without background staining (IF) Used at dilution 1:150 (ICC). Clean strong signal without any apparent cross-reactivity.
Date published: 2018-05-21
Rated 5 out of 5 by from Good staining This antibody works very well at a 1:200 dilution for limmunofluorescence on mouse calvariae.
Date published: 2018-03-16
Rated 5 out of 5 by from Good signal It gave me a very strong signal and barely observed any noises.
Date published: 2018-01-22
Rated 4 out of 5 by from Working well This product products work propertly with my primary antibodies.
Date published: 2018-01-15
Rated 4 out of 5 by from Worked well! We got the sample a month ago and the secondary antibody worked very well!
Date published: 2017-12-19
Rated 5 out of 5 by from VERY GOOD Very good antibody for IF. Dilution 1:100 in IHC analysis in HeLa cells
Date published: 2017-11-17
Rated 5 out of 5 by from Great antibody for IF Good secondary antibody for IF. Fluorescence was easily detectable by LSM710
Date published: 2017-10-29
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