Date published: 2025-10-26

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mouse anti-rabbit IgG-CFL 488: sc-516248

4.7(15)
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Datasheets
  • mouse monoclonal secondary antibody conjugated to CruzFluor™ 488 (CFL 488)
  • supplied at 200 µg in 0.5 ml volume
  • CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, offering comparable photostability and the ability to resist protein quenching
  • Excitation of 488 nm, Emission of 514 nm
  • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
  • Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
  • Highly specific secondary antibody that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with polyclonal secondary antibodies
  • If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-CFL 488 (sc-516248), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-CFL 488 secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.

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Santa Cruz Biotechnology's high quality, well characterized monoclonal secondary antibodies are available conjugated to either an enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, immunostaining and flow cytometry. Santa Cruz secondary antibodies are commonly affinity purified against immobilized whole IgG isotypes, including IgG1, IgG2a, IgG2b, IgG3 and IgG4. Monoclonal secondary antibodies are available conjugated to:
HRP for Western Blotting (WB) and Immunohistochemistry (IHC),
(CM) or Cruz Marker form of HRP conjugated secondary antibodies are suitable for use with our Cruz Marker™ molecular weight standards
FITC (fluorescein isothiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
PE (phycoerythrin) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
B (Biotin) for Immunohistochemistry (IHC)
CruzFluor™ 488, 555, 594 and 647 for RGB Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
CruzFluor™ 680 and 790 for Near-Infrared (NIR) Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM)
AP (alkaline phosphatase) for Western Blotting (WB)
R (TRITC: tetramethyl rhodamine iso-thiocyanate) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
TR (Texas Red®) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunohistochemistry (IHC)
PerCP (peridinin chlorophyll protein complex) and PerCP-Cy5.5 (peridinin chlorophyll protein complex with cyanin-5.5) for Immunofluorescence (IF), Immunohistochemistry (IHC) and Flow Cytometry (FCM).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

mouse anti-rabbit IgG-CFL 488

sc-516248
200 µg/0.5 ml
$77.00

Subject : One more question. Dear Santa Cruz I have another question. The concentration of antibody is 200 µg/0.5 mL, is it precise? Does this sample have higher concentrarion than its notation? Best regards.

Asked by: Tsuji
Thank you for your question.200 µg/0.5 mL is accurate.Thank you.
Answered by: Jiajun Z
Date published: 2024-12-17

Subject : Question Dear Santa Cruz I have a question. Is there any protein in the buffer without the antibody. Best regards. Tsuji.

Asked by: Tsuji
Thank you for your product review.I did not understand your question.Please contact our Technical Service Department of Japan for the consultation optimization suggestion and more information.
Answered by: Jiajun Z
Date published: 2024-12-12
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Rated 5 out of 5 by from Staining HUVECsWorks well as a secondary antibody (1:50 dilution) for HUVECs!
Date published: 2024-10-30
Rated 5 out of 5 by from The item from Santa Cruz, anti-rabbit IgG-CFL 488,The item from Santa Cruz, anti-rabbit IgG-CFL 488, is good.
Date published: 2024-09-11
Rated 5 out of 5 by from 功能出色6个月前购买了mouse IgG结合二抗,效果显著。488/594均可在1:200倍比稀释情况下,具有良好的结合效果。
Date published: 2022-08-12
Rated 5 out of 5 by from Good signal!!Very nice signal diluition 1:200 for neural stem cell derived astrocytes
Date published: 2020-07-22
Rated 5 out of 5 by from IF-C 1:200Since I started using this secondary antibody, I was not bothered by nonspecific reactions.
Date published: 2019-07-05
Rated 5 out of 5 by from Strong signal without background staining (IF)Used at dilution 1:150 (ICC). Clean strong signal without any apparent cross-reactivity.
Date published: 2018-05-21
Rated 5 out of 5 by from Good stainingThis antibody works very well at a 1:200 dilution for limmunofluorescence on mouse calvariae.
Date published: 2018-03-16
Rated 5 out of 5 by from Good signalIt gave me a very strong signal and barely observed any noises.
Date published: 2018-01-22
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mouse anti-rabbit IgG-CFL 488 is rated 4.7 out of 5 by 15.
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