monodisperse Mal-PEG₄-NHS-ester (CAS 756525-99-2)

Monodisperse Mal-PEG4-NHS-ester is a chemical compound that plays a critical role in the field of bioconjugation, particularly in the creation of highly specific and stable linkages between biomolecules for various research applications. This compound features three key functional groups: a maleimide (Mal) group, a polyethylene glycol (PEG) chain consisting of four ethylene glycol units (PEG4), and an N-hydroxysuccinimide (NHS) ester. The maleimide group is noted for its ability to form covalent bonds with thiol groups, such as those found on the cysteine residues of proteins, without affecting the overall structure of the protein. This specificity makes it extremely valuable for targeting and modifying specific sites within proteins. The NHS ester enhances the molecule′s ability to react with primary amine groups, typically found at the N-terminus of proteins or on lysine side chains, facilitating the formation of stable amide bonds. The inclusion of a PEG4 spacer not only increases the hydrophilicity and biocompatibility of the conjugate but also reduces aggregation and immunogenicity, making the conjugates more suitable for in vitro and in vivo studies. Researchers utilize monodisperse Mal-PEG4-NHS-ester in designing and synthesizing protein conjugates, peptides, and other molecular constructs in drug delivery research, diagnostic assays, and material science, where precision and stability of the conjugates are paramount. This compound′s monodisperse nature ensures uniformity and reproducibility in biochemical reactions, crucial for high-quality research outcomes.