MLF1IP CRISPR/Cas9 KO Plasmid (h): sc-413208
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MLF1IP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MLF1IP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLF1IP CRISPR/Cas9 KO Plasmid (h) | sc-413208 | 20 µg | $397.00 |
Overview
CENPU encodes the centromere protein U (MLF1IP), a core component of the CENP-O/P/Q/U complex required for proper kinetochore assembly and stable microtubule attachment during mitosis. MLF1IP supports accurate chromosome congression and segregation, helping maintain genomic stability through orderly progression of the cell cycle and spindle checkpoint-associated processes. Disruption or dysregulation of centromere/kinetochore factors can drive chromosome missegregation and aneuploidy, linking CENPU function to mechanisms commonly associated with proliferative disease phenotypes. As a mitotic regulator, CENPU is frequently studied in the context of cell division control, chromosomal instability pathways, and cancer-related cell cycle remodeling.
MLF1IP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CENPU gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CENPU together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CENPU open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MLF1IP protein expression.
This CRISPR knockout system enables efficient generation of CENPU-deficient cell models for investigation of MLF1IP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CENPU exon(s) critical for MLF1IP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CENPU genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MLF1IP CRISPR/Cas9 KO Plasmid (h) and MLF1IP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CENPU locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MLF1IP HDR Plasmid (h) and MLF1IP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CENPU homology arms to support homology-directed repair at defined CENPU target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.