LMX1B Double Nickase Plasmid (h): sc-402945-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LMX1B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LMX1B Double Nickase Plasmid (h) and LMX1B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LMX1B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LMX1B Antibody (1D12): sc-293262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LMX1B Double Nickase Plasmid (h) | sc-402945-NIC | 20 µg | $410.00 | |||
LMX1B Double Nickase Plasmid (h2) | sc-402945-NIC-2 | 20 µg | $410.00 |
LMX1B encodes a LIM homeobox transcription factor that regulates cell fate specification and tissue patterning during development, with prominent roles in limb dorsalization, kidney podocyte differentiation, and neuronal subtype identity. Through LIM-domain cofactor interactions and DNA-binding via its homeodomain, LMX1B coordinates transcriptional programs that influence extracellular matrix organization, cytoskeletal dynamics, and morphogen signaling networks. Genetic disruption of LMX1B is linked to Nail–patella syndrome and is associated with podocyte dysfunction and renal manifestations, making it a relevant target for studying developmental gene regulation and glomerular biology. In addition, altered LMX1B expression has been investigated in the context of cancer cell lineage states and transcriptional network rewiring.
LMX1B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LMX1B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LMX1B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LMX1B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LMX1B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.