LBP Antibody (4E8): sc-293253

LBP Antibody (4E8) is a mouse monoclonal IgM antibody that detects LBP protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-LBP antibody (4E8) is available as the non-conjugated format. Lipopolysaccharide-binding protein (LBP) plays a crucial role in the immune response, particularly in the context of Gram-negative bacterial infections. LBP is primarily located in the serum and is secreted by hepatic and intestinal epithelial cells during inflammatory responses. LBP binds to lipopolysaccharides (LPS), which are components of the outer membrane of Gram-negative bacteria, facilitating their recognition by the immune system. This binding is essential for the activation of immune cells, as LBP enhances the transfer of LPS to CD14 receptors on macrophages, thereby amplifying the inflammatory response. LBP includes a 25-residue signal sequence followed by a 452-residue mature protein, which contains four cysteine residues and five potential glycosylation sites, contributing to stability and function. Elevated levels of LBP are associated with a heightened sensitivity to endotoxins, which can protect against sepsis, underscoring the importance of LBP in maintaining immune homeostasis during infections.

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LBP Antibody (4E8) References:

1. Membrane-anchored forms of lipopolysaccharide (LPS)-binding protein do not mediate cellular responses to LPS independently of CD14.  |  Tapping, RI., et al. 1999. J Immunol. 162: 5483-9. PMID: 10228028
2. The transcriptional factor LBP-1c/CP2/LSF gene on chromosome 12 is a genetic determinant of Alzheimer's disease.  |  Lambert, JC., et al. 2000. Hum Mol Genet. 9: 2275-80. PMID: 11001930
3. Lipopolysaccharide-binding protein is vectorially secreted and transported by cultured intestinal epithelial cells and is present in the intestinal mucus of mice.  |  Vreugdenhil, AC., et al. 2000. J Immunol. 165: 4561-6. PMID: 11035097
4. Association of the 3' UTR transcription factor LBP-1c/CP2/LSF polymorphism with late-onset Alzheimer's disease.  |  Luedecking-Zimmer, E., et al. 2003. Am J Med Genet B Neuropsychiatr Genet. 117B: 114-7. PMID: 12555245
5. Heterodimerization with LBP-1b is necessary for nuclear localization of LBP-1a and LBP-1c.  |  Sato, F., et al. 2005. Genes Cells. 10: 861-70. PMID: 16115195
6. Association of polymorphism in the transcription factor LBP-1c/CP2/LSF gene with Alzheimer's disease and major depression.  |  Schahab, S., et al. 2006. Dement Geriatr Cogn Disord. 22: 95-8. PMID: 16710089
7. TFCP2c/LSF/LBP-1c is required for Snail1-induced fibronectin gene expression.  |  Porta-de-la-Riva, M., et al. 2011. Biochem J. 435: 563-8. PMID: 21361876
8. Structure and function of lipopolysaccharide binding protein.  |  Schumann, RR., et al. 1990. Science. 249: 1429-31. PMID: 2402637
9. TFCP2/TFCP2L1/UBP1 transcription factors in cancer.  |  Kotarba, G., et al. 2018. Cancer Lett. 420: 72-79. PMID: 29410248
10. Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity.  |  Volker, JL., et al. 1997. Genes Dev. 11: 1435-46. PMID: 9192871
11. Lipopolysaccharide-binding protein is required to combat a murine gram-negative bacterial infection.  |  Jack, RS., et al. 1997. Nature. 389: 742-5. PMID: 9338787
12. Neutrophils responded to immobilized lipopolysaccharide in the absence of lipopolysaccharide-binding protein.  |  Nakatomi, K., et al. 1998. J Leukoc Biol. 64: 177-84. PMID: 9715256