LARP4 CRISPR/Cas9 KO Plasmid (h): sc-414019
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LARP4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LARP4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LARP4 CRISPR/Cas9 KO Plasmid (h) | sc-414019 | 20 µg | $397.00 |
Overview
La ribonucleoprotein domain family member 4 (LARP4) is an RNA-binding protein that associates with poly(A)-binding protein and regulates mRNA stability and translation efficiency in the cytoplasm. Through interactions with mRNP complexes and translation machinery, LARP4 influences post-transcriptional gene regulation, affecting protein output and cellular adaptation to growth and stress cues. These functions connect LARP4 to pathways controlling cell proliferation, migration, and cytoskeletal dynamics via downstream changes in transcript fate and translational control. Dysregulated RNA metabolism and translation are recurrent features of multiple cancers and neurodevelopmental contexts, making LARP4 a useful node for mechanistic studies of disease-relevant gene expression programs.
LARP4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LARP4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LARP4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LARP4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LARP4 protein expression.
This CRISPR knockout system enables efficient generation of LARP4-deficient cell models for investigation of LARP4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LARP4 exon(s) critical for LARP4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LARP4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LARP4 CRISPR/Cas9 KO Plasmid (h) and LARP4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LARP4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LARP4 HDR Plasmid (h) and LARP4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LARP4 homology arms to support homology-directed repair at defined LARP4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.