KCTD3 CRISPR/Cas9 KO Plasmid (h): sc-412124
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCTD3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KCTD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCTD3 CRISPR/Cas9 KO Plasmid (h) | sc-412124 | 20 µg | $397.00 |
Overview
KCTD3 (potassium channel tetramerization domain containing 3) encodes a BTB/POZ domain–containing protein implicated in protein–protein interactions that can shape the assembly of multiprotein complexes and influence proteostasis. Members of the KCTD family are frequently linked to cullin-3 (CUL3)–associated ubiquitin ligase biology and to the regulation of signaling intermediates through targeted turnover, suggesting roles in pathway tuning and cellular homeostasis. Through these interaction networks, KCTD3 is relevant to studies of cell growth control, stress responses, and differentiation-associated programs where ubiquitination-dependent remodeling is prominent. Dysregulation of BTB/KCTD-centered complexes has been associated with disease-relevant phenotypes in cancer and neurobiology, supporting investigation of KCTD3 in mechanistic models of altered signaling and protein quality control.
KCTD3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCTD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCTD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCTD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KCTD3 protein expression.
This CRISPR knockout system enables efficient generation of KCTD3-deficient cell models for investigation of KCTD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCTD3 exon(s) critical for KCTD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCTD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KCTD3 CRISPR/Cas9 KO Plasmid (h) and KCTD3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCTD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KCTD3 HDR Plasmid (h) and KCTD3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCTD3 homology arms to support homology-directed repair at defined KCTD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.