Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin β1 Double Nickase Plasmid (h): sc-401230-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin β1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- karyopherin β1 Double Nickase Plasmid (h) and karyopherin β1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KPNB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin β1 Antibody (H-7): sc-137016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin β1 Double Nickase Plasmid (h) | sc-401230-NIC | 20 µg | $410.00 | |||
karyopherin β1 Double Nickase Plasmid (h2) | sc-401230-NIC-2 | 20 µg | $410.00 |
KPNB1 encodes karyopherin β1 (importin-β1), a core nuclear transport receptor that mediates RanGTP-dependent import of proteins bearing classical nuclear localization signals in cooperation with importin-α adaptors. By regulating nucleocytoplasmic trafficking of transcription factors, DNA repair proteins, and cell-cycle regulators, KPNB1 influences gene expression programs, checkpoint control, and stress-responsive signaling. This pathway is integral to mitotic progression and proteostasis because selective nuclear import coordinates replication, transcription, and ribonucleoprotein assembly. Altered nuclear transport dynamics and dysregulated KPNB1 activity have been linked to proliferative and stress-adaptation phenotypes, making it a useful target for studies of oncogenic signaling, genome maintenance, and host–pathogen interactions.
karyopherin β1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KPNB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KPNB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KPNB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KPNB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.