Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h): sc-400380-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h) and Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGA6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α6/ITGA6/CD49f Antibody (F-6): sc-374057
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h) | sc-400380-NIC | 20 µg | $410.00 | |||
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h2) | sc-400380-NIC-2 | 20 µg | $410.00 |
ITGA6 encodes integrin α6 (CD49f), an α subunit that pairs primarily with β1 or β4 to form laminin-binding receptors that mediate cell–extracellular matrix adhesion. Integrin α6 complexes coordinate focal adhesion and hemidesmosome-associated signaling to regulate epithelial polarity, migration, and survival through pathways including FAK/SRC, PI3K–AKT, and MAPK. CD49f is widely used as a marker associated with epithelial progenitors and stem-like compartments, reflecting its role in maintaining tissue architecture and niche interactions. Dysregulated ITGA6 expression or altered integrin signaling is linked to invasion, metastatic dissemination, and therapy resistance phenotypes in multiple tumor contexts, as well as basement-membrane and skin blistering biology via α6β4-dependent anchoring structures.
Integrin α6/ITGA6/CD49f Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGA6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGA6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGA6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGA6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.