Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β1 Antibody (E-11): sc-374430. Western blot analysis of Integrin β1 expression in A549 (A), NIH/3T3 (B), C2C12 (C), C6 (D), A-431 (E) and U-2 OS (F) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin β1/ITGB1 Double Nickase Plasmid (m): sc-421171-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β1/ITGB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β1/ITGB1 Double Nickase Plasmid (m) and Integrin β1/ITGB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itgb1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β1/ITGB1 Antibody (A-4): sc-374429
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β1/ITGB1 Double Nickase Plasmid (m) | sc-421171-NIC | 20 µg | $410.00 | |||
Integrin β1/ITGB1 Double Nickase Plasmid (m2) | sc-421171-NIC-2 | 20 µg | $410.00 |