Molecular structure of Hygromycin B, CAS Number: 31282-04-9
Hygromycin B (CAS 31282-04-9)
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Alternate Names:
O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
CAS Number:
31282-04-9
Molecular Weight:
527.5
Molecular Formula:
C20H37N3O13
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Hygromycin B is an aminoglycoside antibiotic substance naturally produced by Streptomyces hygroscopicus, which also shows anthelmintic activity. Hygromycin B may act to effectively inhibit translocation of mRNA and tRNAs on the ribosome in both bacteria and eukaryotes and also has a subtle effect on decoding fidelity as it slightly increases aminoacyl tRNA (A-site tRNA) affinity. Hygromycin B is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that Hygromycin B binds to a single site on the ribosome.
Hygromycin B (CAS 31282-04-9) References
- The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. | Brodersen, DE., et al. 2000. Cell. 103: 1143-54. PMID: 11163189
- Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. | Olhoft, PM., et al. 2003. Planta. 216: 723-35. PMID: 12624759
- Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. | Pfister, P., et al. 2003. Antimicrob Agents Chemother. 47: 1496-502. PMID: 12709313
- Aminoglycoside and aminocyclitol antibiotics: hygromycin B is an atypical bactericidal compound that exerts effects on cells of Escherichia coli characteristics for bacteriostatic aminocyclitols. | Bakker, EP. 1992. J Gen Microbiol. 138: 563-9. PMID: 1375624
- Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. | Punt, PJ. and van den Hondel, CA. 1992. Methods Enzymol. 216: 447-57. PMID: 1479914
- Structural basis for hygromycin B inhibition of protein biosynthesis. | Borovinskaya, MA., et al. 2008. RNA. 14: 1590-9. PMID: 18567815
- Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition. | Cabañas, MJ., et al. 1978. Eur J Biochem. 87: 21-7. PMID: 208837
- Structure and function of APH(4)-Ia, a hygromycin B resistance enzyme. | Stogios, PJ., et al. 2011. J Biol Chem. 286: 1966-75. PMID: 21084294
- Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. | Radman, I., et al. 2013. PLoS One. 8: e76019. PMID: 24130756
- Resistance to hygromycin B : A new marker for plant transformation studies. | Waldron, C., et al. 1985. Plant Mol Biol. 5: 103-8. PMID: 24306569
- Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. | González, A., et al. 1978. Biochim Biophys Acta. 521: 459-69. PMID: 367435
- Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. | Santerre, RF., et al. 1984. Gene. 30: 147-56. PMID: 6096211
- Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. | Kaster, KR., et al. 1983. Nucleic Acids Res. 11: 6895-911. PMID: 6314265
- Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. | Rao, RN., et al. 1983. Antimicrob Agents Chemother. 24: 689-95. PMID: 6318654
- Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals. | Mach, RL., et al. 1994. Curr Genet. 25: 567-70. PMID: 8082210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hygromycin B, 1 g | sc-506168 | 1 g | $156.00 | |||
Hygromycin B, 25 g | sc-506168A | 25 g | $1109.00 | |||
Hygromycin B, 100 g | sc-506168B | 100 g | $3029.00 |