HoxB2 Double Nickase Plasmid (h): sc-404533-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxB2 Double Nickase Plasmid (h) and HoxB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXB2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB2 Double Nickase Plasmid (h) | sc-404533-NIC | 20 µg | $410.00 | |||
HoxB2 Double Nickase Plasmid (h2) | sc-404533-NIC-2 | 20 µg | $410.00 |
HOXB2 encodes the homeobox transcription factor HoxB2, a sequence-specific DNA-binding regulator that patterns the anterior–posterior axis during embryogenesis and supports segmentation and differentiation programs in the developing hindbrain and neural crest–derived tissues. As part of HOX gene regulatory networks, HoxB2 coordinates transcriptional programs controlling lineage specification, cell identity maintenance, and morphogen-responsive developmental pathways, including retinoic acid–dependent patterning. Dysregulated HOXB2 expression has been reported across multiple tumor types and is studied for roles in aberrant differentiation states, invasive behavior, and transcriptional rewiring in cancer models. In human cell systems, HOXB2 perturbation is commonly used to interrogate developmental gene regulatory circuits and disease-relevant changes in cell fate.
HoxB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.