HEPPSO sodium salt (CAS 89648-37-3)

HEPPSO sodium salt is a zwitterionic buffer compound derived from the parent structure HEPPSO (N-2-hydroxyethylpiperazine-N′-2-propanesulfonic acid), tailored for use in biological and chemical research, particularly in environments requiring precise pH control. This sodium salt form enhances the solubility and buffering capabilities of HEPPSO, making it particularly effective in the pH range of 7.3 to 8.7. The key mechanism of action for HEPPSO sodium salt lies in its ability to stabilize pH by absorbing excess hydrogen ions when the environment becomes too acidic, and releasing them when conditions become too alkaline. This dynamic ion exchange helps maintain a consistent pH, which is crucial for maintaining the structural integrity and functionality of sensitive biomolecules during experimental procedures. In research, HEPPSO sodium salt is widely used in studies involving enzyme kinetics and other biochemical reactions where the pH must be strictly controlled to ensure accurate and reproducible results. Its buffering properties are especially valuable in protein purification processes and electrophoresis, where maintaining a stable pH ensures the proteins′ native charges and shapes are preserved, facilitating accurate separation and analysis. Additionally, the low ionic strength and minimal interference with biological systems make HEPPSO sodium salt a preferred choice for researchers working in molecular biology and biotechnology fields, where even minor changes in pH can significantly impact the outcomes of experiments.