Date published: 2025-9-5
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HA-Tag 抗体 (153): sc-53516

4.6(9)
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说明书
  • HA-Tag 抗体 153 是大鼠单克隆 IgG2a, 在25篇文献中引用，提供 200 µg/ml
  • 针对流感血凝素衍生的HA肽
  • 推荐用于 proteins containing the HA tag WB, IP, IF 和 FCM检测
  • 另提供Alexa Fluor® 647 偶联抗体，可用于IF、IHC(P)和FCM实验
  • 目前，我们还没有完成HA-Tag 抗体 (153)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
说明书与实验方案
研究信息

関連項目

此外 HA 抗体包括 HA-probe

HA-Tag抗体（153）是一种鼠单克隆IgG2a抗体，针对HA肽（源自流感血凝素蛋白）产生。HA-Tag是一种11个氨基酸的序列，通常与目标蛋白融合，用于在细菌、昆虫和哺乳动物细胞等各种系统中进行纯化、检测和定位研究。HA标签蛋白的细胞定位对于理解生物学功能至关重要，因为定位通常决定了相互作用、信号通路中的角色以及参与细胞过程。HA-Tag (153)抗体能够检测这些蛋白，无论它们位于细胞质、细胞核还是细胞膜中，帮助研究人员探索动态细胞事件。血凝素通过与宿主细胞膜结合在病毒进入过程中发挥作用，其结构确保了HA标签特异性表位的可及性。这种特异性使得在应用中可以观察和追踪标记的蛋白质，例如石蜡包埋切片免疫组织化学（IHCP）和免疫荧光（IF），这取决于识别蛋白质所在的精确细胞区室。这些研究对于阐明蛋白质功能和调节细胞过程的机制至关重要。HA-Tag (153) 单克隆抗体有非结合型和结合型两种形式，为蛋白质研究中的各种实验设计提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

HA-Tag 抗体 (153)

sc-53516
200 µg/ml
$316.00

HA-probe 抗体 (153) Alexa Fluor® 488

sc-53516 AF488
200 µg/ml
$357.00

What is the dilution that should be used for IF?

Asked by: IF AB
Thank you for your question. We recommend a starting dilution of 1:50-1:500. Here is our protocol:https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2022-11-14

What is the species reactivity of this antibody?

Asked by: AbPolly
Thank you for your question. This HA-probe antibody can be used to detect HA-tagged proteins in samples from any species, the reactivity is not species specific.
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 1 out of 5 by from Do not use this AbDo not use this Ab. It doesn't work for WB and IF. But, sc-7392 works well. Buy sc-7392 but not this Ab.
Date published: 2017-06-06
Rated 5 out of 5 by from Good HA antibodyHA-probe (153) has positive customer feedback for WB, IF, and IHC(P).
Date published: 2016-12-22
Rated 5 out of 5 by from Good qualityI use this antibody in western blot and got ideal bands , it has high specificity and the background is very clear!
Date published: 2016-11-04
Rated 5 out of 5 by from Able to publish using the antibody in WBAble to publish using the antibody in WB in human sample.
Date published: 2015-09-23
Rated 5 out of 5 by from Works very nicely for IF on mouse samplesWorks very nicely for IF on mouse samples. -SCBT Publication Review
Date published: 2015-08-05
Rated 5 out of 5 by from No commentNo comment
Date published: 2015-03-31
Rated 5 out of 5 by from Good reliable monoGood reliable mono, used many times. It's my groups first choice for HA-probe. -SCBT QC
Date published: 2014-12-17
Rated 5 out of 5 by from Used for IHC and WB on our rat samplesUsed for IHC and WB on our rat samples. -SCBT Publication Review
Date published: 2014-11-04
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HA-Tag 抗体 (153) is rated 4.6 out of 5 by 9.
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