Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR43 CRISPR/Cas9 KO Plasmid (h): sc-401858
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR43 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR43 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR43 Antibody (3B3): sc-293202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR43 CRISPR/Cas9 KO Plasmid (h) | sc-401858 | 20 µg | $397.00 |
Overview
FFAR2 encodes GPR43, a short-chain fatty acid (SCFA)–sensing G protein-coupled receptor activated by acetate, propionate, and butyrate produced by the gut microbiota. GPR43 couples primarily to Gαi/o and Gαq pathways to modulate cAMP signaling, intracellular calcium flux, and downstream MAPK/ERK responses, shaping chemotaxis, cytokine production, and barrier-associated immune regulation. It is prominently expressed in immune cells such as neutrophils and macrophages and in metabolic tissues, linking microbial metabolites to inflammatory and energy homeostasis programs. Dysregulated FFAR2/GPR43 signaling has been associated with inflammatory disorders and metabolic phenotypes, supporting mechanistic studies of host–microbiome signaling in disease-relevant contexts.
GPR43 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FFAR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FFAR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FFAR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR43 protein expression.
This CRISPR knockout system enables efficient generation of FFAR2-deficient cell models for investigation of GPR43 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FFAR2 exon(s) critical for GPR43 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FFAR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR43 CRISPR/Cas9 KO Plasmid (h) and GPR43 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FFAR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR43 HDR Plasmid (h) and GPR43 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FFAR2 homology arms to support homology-directed repair at defined FFAR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.