GOLGB1 Double Nickase Plasmid (m): sc-432441-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GOLGB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GOLGB1 Double Nickase Plasmid (m) and GOLGB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Golgb1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GOLGB1 Double Nickase Plasmid (m) | sc-432441-NIC | 20 µg | $410.00 |
Golgb1 encodes the golgin family scaffold protein GOLGB1 (giantin), a large coiled-coil component of the Golgi matrix that contributes to maintaining Golgi ribbon architecture and organizing vesicle tethering. GOLGB1 interfaces with trafficking pathways that support ER-to-Golgi transport, intra-Golgi cargo progression, and glycosylation-dependent maturation of secretory and membrane proteins. Through its role in Golgi homeostasis, Golgb1 influences processes such as extracellular matrix deposition, receptor surface expression, and polarized secretion that are central to development and tissue organization. Perturbation of Golgi structural proteins, including giantin, has been associated with defects in ciliogenesis and connective tissue biology, making Golgb1 a useful target for studying organelle-dependent mechanisms relevant to developmental phenotypes.
GOLGB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Golgb1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Golgb1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Golgb1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Golgb1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.