GnRHR Double Nickase Plasmid (h): sc-401783-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GnRHR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GnRHR Double Nickase Plasmid (h) and GnRHR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNRHR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GnRHR Antibody (GRX-5): sc-69845
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GnRHR Double Nickase Plasmid (h) | sc-401783-NIC | 20 µg | $410.00 | |||
GnRHR Double Nickase Plasmid (h2) | sc-401783-NIC-2 | 20 µg | $410.00 |
GNRHR encodes the human gonadotropin-releasing hormone receptor (GnRHR), a rhodopsin-like G protein-coupled receptor predominantly expressed in pituitary gonadotrophs. Upon binding hypothalamic GnRH, GnRHR couples mainly to Gαq/11 to activate phospholipase Cβ, increasing IP3/DAG signaling, intracellular Ca2+ mobilization, and PKC/MAPK pathway activity to regulate transcription and secretion of luteinizing hormone and follicle-stimulating hormone. This signaling axis integrates pulsatile endocrine inputs to control reproductive development and fertility, and perturbations in GNRHR function are linked to disorders of the hypothalamic–pituitary–gonadal axis such as congenital hypogonadotropic hypogonadism and related reproductive endocrine phenotypes. In cellular models, GnRHR provides a tractable node for studying GPCR desensitization, β-arrestin-dependent trafficking, and stimulus-frequency decoding.
GnRHR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNRHR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNRHR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNRHR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNRHR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.