GluR-3 Double Nickase Plasmid (m): sc-424739-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GluR-3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GluR-3 Double Nickase Plasmid (m) and GluR-3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Gria3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GluR-3 Antibody (1D2E2): sc-517202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GluR-3 Double Nickase Plasmid (m) | sc-424739-NIC | 20 µg | $410.00 | |||
GluR-3 Double Nickase Plasmid (m2) | sc-424739-NIC-2 | 20 µg | $410.00 |
Gria3 encodes the AMPA-type glutamate receptor subunit GluR-3, a ligand-gated ion channel that mediates fast excitatory neurotransmission in the mouse central nervous system. GluR-3-containing receptors regulate synaptic transmission and plasticity through activity-dependent trafficking and phosphorylation, shaping processes such as long-term potentiation and dendritic spine remodeling. By controlling Na\+/K\+ conductance and downstream Ca2\+-dependent signaling, GRIA3 intersects with pathways including MAPK/ERK, CAMKII, and immediate-early gene programs that couple neuronal activity to transcriptional responses. Altered AMPA receptor composition or signaling has been implicated in neurodevelopmental and neuropsychiatric phenotypes as well as excitotoxic stress responses, making Gria3 a useful target for mechanistic studies of circuit function.
GluR-3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Gria3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gria3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gria3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gria3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.