Gemin3 Antibody (2): sc-135919
- Gemin3 Antibody (2) is a mouse monoclonal IgG2b provided at 50 µg/500 µl
- raised against amino acids 667-783 of Gemin3 of human origin
- recommended for detection of Gemin3 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Gemin3 Antibody (2). This work is in progress.
Gemin3 Antibody (2) is a mouse monoclonal IgG2b antibody that detects Gemin3 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. Gemin3, also known as DP103, DDX20, and DEAD-box protein DP130, is a crucial component of the survival of motor neuron (SMN) complex, which is essential for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) in the cytoplasm and their subsequent role in pre-mRNA splicing within the nucleus. Gemin3 is located in both the cytoplasm and the nucleus, with a significant concentration in subnuclear structures known as gems, which are vital for proper RNA processing. Gemin3′s presence in these cellular compartments underscores its importance in maintaining RNA metabolism and gene expression regulation. Additionally, Gemin3 interacts with several other proteins, including SmB, SmD2, and SmD3, and possesses ATPase activity, indicating a potential role as a putative RNA helicase. Gemin3 expression is observed in various tissues, including B and T cell neuroblastoma-derived cell lines, malignant melanoma, and normal testis, while showing lower levels in colon, skeletal muscle, liver, kidney, and lung tissues. Anti-Gemin3 antibody (2) effectively targets this essential protein, making Gemin3 (2) monoclonal antibody a valuable tool for researchers studying molecular mechanisms underlying spinal muscular atrophy and other related disorders.
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Gemin3 Antibody (2) References:
- Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. | Charroux, B., et al. 1999. J Cell Biol. 147: 1181-94. PMID: 10601333
- The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification. | Lee, MB., et al. 2005. Mol Cell Biol. 25: 1879-90. PMID: 15713642
- Abnormal interaction of motor neuropathy-associated mutant HspB8 (Hsp22) forms with the RNA helicase Ddx20 (gemin3). | Sun, X., et al. 2010. Cell Stress Chaperones. 15: 567-82. PMID: 20157854
- Genetic variants in the MicroRNA biosynthetic pathway Gemin3 and Gemin4 are associated with a risk of cancer: a meta-analysis. | Zhu, W., et al. 2016. PeerJ. 4: e1724. PMID: 27019773
- The multiple lives of DEAD-box RNA helicase DP103/DDX20/Gemin3. | Curmi, F. and Cauchi, RJ. 2018. Biochem Soc Trans. 46: 329-341. PMID: 29523774
- SMN complex member Gemin3 self-interacts and has a functional relationship with ALS-linked proteins TDP-43, FUS and Sod1. | Cacciottolo, R., et al. 2019. Sci Rep. 9: 18666. PMID: 31822699
- The combined detection of Amphiregulin, Cyclin A1 and DDX20/Gemin3 expression predicts aggressive forms of oral squamous cell carcinoma. | Bourova-Flin, E., et al. 2021. Br J Cancer. 125: 1122-1134. PMID: 34290392
- Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons. | Miralles, MP., et al. 2022. Front Cell Neurosci. 16: 1054270. PMID: 36619669
- Single nucleotide polymorphisms of GEMIN3 modify the risk of primary Sjögren's syndrome in female patients. | Wang, D., et al. 2025. Am J Med Sci. 369: 137-144. PMID: 38969288
- A critical genetic interaction between Gemin3/Ddx20 and translation initiation factor NAT1/eIF4G2 drives development. | Cacciottolo, R. and Cauchi, RJ. 2025. Dev Biol. 521: 37-51. PMID: 39924071
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gemin3 Antibody (2) | sc-135919 | 50 µg/500 µl | $322.00 |