GCIP Antibody (C-9): sc-514518
- GCIP Antibody (C-9) is a mouse monoclonal IgG1 κ GCIP antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 140-156 within an internal region of GCIP of human origin
- GCIP Antibody (C-9) is recommended for detection of GCIP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GCIP Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for GCIP Antibody (C-9) for WB applications. This reagent is now offered in a bundle with GCIP Antibody (C-9) (see ordering information below).
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GCIP Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects GCIP of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCIP (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCIP, also known as cyclin D-type binding-protein 1 (CCNDBP1), human homolog of maid (HHM), or DIP1, is a 360 amino acid protein located in both the cytoplasm and nucleus, belonging to the CCNDBP1 family. This dual localization enables GCIP to participate in various cellular processes, including gene expression regulation and cell cycle progression. GCIP interacts with cyclin D and Gads, a leukocyte-specific adaptor protein that plays a significant role in immune cell signaling. As a negative regulator of liver-specific gene expression, GCIP inhibits retinoblastoma protein phosphorylation by interfering with the cyclin D/Cdk4 complex, thereby influencing cell cycle dynamics. GCIP expression is down-regulated in several tumors, including rectum, breast, prostate, and colon, while up-regulated in hepatic cancers, highlighting a potential role in tumorigenesis. GCIP exists as at least four alternatively spliced isoforms, with expression levels increasing during differentiation and being inducible by sodium butyrate, further emphasizing importance in cellular regulation and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GCIP Antibody (C-9) References:
- GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity. | Xia, C., et al. 2000. J Biol Chem. 275: 20942-8. PMID: 10801854
- Expression of GCIP in transgenic mice decreases susceptibility to chemical hepatocarcinogenesis. | Ma, W., et al. 2006. Oncogene. 25: 4207-16. PMID: 16501603
- Human GCIP interacts with CT847, a novel Chlamydia trachomatis type III secretion substrate, and is degraded in a tissue-culture infection model. | Chellas-Géry, B., et al. 2007. Cell Microbiol. 9: 2417-30. PMID: 17532760
- Ribosomal phosphoprotein P0 interacts with GCIP and overexpression of P0 is associated with cellular proliferation in breast and liver carcinoma cells. | Chang, TW., et al. 2008. Oncogene. 27: 332-8. PMID: 17621266
- An Id-like molecule, HHM, is a synexpression group-restricted regulator of TGF-beta signalling. | Ikushima, H., et al. 2008. EMBO J. 27: 2955-65. PMID: 18923419
- Immunohistochemical expression of GCIP in breast carcinoma: relationship with tumour grade, disease-free survival, mucinous differentiation and response to chemotherapy. | Chen, WC., et al. 2008. Histopathology. 53: 554-60. PMID: 18983464
- Crystallization and preliminary X-ray diffraction analysis of GCIP/HHM transcriptional regulator. | Seto, A., et al. 2009. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65: 21-4. PMID: 19153449
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCIP Antibody (C-9) | sc-514518 | 200 µg/ml | $322.00 | |||
GCIP Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-531786 | 200 µg Ab; 20 µg BP | $361.00 | |||
GCIP Antibody (C-9) AC | sc-514518 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GCIP Antibody (C-9) HRP | sc-514518 HRP | 200 µg/ml | $322.00 | |||
GCIP Antibody (C-9) FITC | sc-514518 FITC | 200 µg/ml | $336.00 | |||
GCIP Antibody (C-9) PE | sc-514518 PE | 200 µg/ml | $349.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 488 | sc-514518 AF488 | 200 µg/ml | $364.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 546 | sc-514518 AF546 | 200 µg/ml | $364.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 594 | sc-514518 AF594 | 200 µg/ml | $364.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 647 | sc-514518 AF647 | 200 µg/ml | $364.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 680 | sc-514518 AF680 | 200 µg/ml | $364.00 | |||
GCIP Antibody (C-9) Alexa Fluor® 790 | sc-514518 AF790 | 200 µg/ml | $364.00 | |||
GCIP (C-9) Neutralizing Peptide | sc-514518 P | 100 µg/0.5 ml | $69.00 |