FRP-1 CRISPR/Cas9 KO Plasmid (m): sc-422897
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FRP-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FRP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FRP-1 CRISPR/Cas9 KO Plasmid (m) | sc-422897 | 20 µg | $397.00 |
Overview
Sfrp1 encodes secreted frizzled-related protein 1 (FRP-1), a secreted modulator of Wnt signaling that binds Wnt ligands and Frizzled receptors to shape pathway amplitude and spatial gradients. By tuning canonical β-catenin–dependent transcription and non-canonical Wnt outputs, FRP-1 influences cell proliferation, differentiation, migration, and extracellular matrix remodeling during development and tissue homeostasis. In mouse models and mechanistic studies, altered Sfrp1 activity has been linked to dysregulated Wnt pathway states relevant to fibrosis, metabolic and skeletal phenotypes, and tumor-associated signaling contexts. Because Sfrp1 functions at the extracellular interface, it is frequently used to dissect autocrine versus paracrine control of Wnt-driven programs.
FRP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sfrp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sfrp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sfrp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FRP-1 protein expression.
This CRISPR knockout system enables efficient generation of Sfrp1-deficient cell models for investigation of FRP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sfrp1 exon(s) critical for FRP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sfrp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FRP-1 CRISPR/Cas9 KO Plasmid (m) and FRP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sfrp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FRP-1 HDR Plasmid (m) and FRP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sfrp1 homology arms to support homology-directed repair at defined Sfrp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.