frizzled-7 CRISPR/Cas9 KO Plasmid (m): sc-420437
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- frizzled-7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the frizzled-7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
frizzled-7 CRISPR/Cas9 KO Plasmid (m) | sc-420437 | 20 µg | $397.00 |
Overview
Mouse Fzd7 encodes frizzled-7, a seven-transmembrane Wnt receptor that transduces extracellular Wnt ligands into intracellular signaling outputs. Frizzled-7 regulates β-catenin–dependent transcriptional programs as well as non-canonical planar cell polarity and Wnt/Ca²⁺ pathways, shaping proliferation, polarity, migration, and fate decisions during development and tissue homeostasis. Altered Fzd7 signaling has been linked to dysregulated stem/progenitor cell behavior and oncogenic pathway activation in multiple experimental models, making it a useful entry point for dissecting Wnt network control. In cell systems, Fzd7 perturbation is commonly used to interrogate receptor-level Wnt signaling specificity and crosstalk with receptor tyrosine kinase and Hippo/YAP-associated processes.
frizzled-7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fzd7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fzd7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fzd7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish frizzled-7 protein expression.
This CRISPR knockout system enables efficient generation of Fzd7-deficient cell models for investigation of frizzled-7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fzd7 exon(s) critical for frizzled-7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fzd7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by frizzled-7 CRISPR/Cas9 KO Plasmid (m) and frizzled-7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fzd7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by frizzled-7 HDR Plasmid (m) and frizzled-7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fzd7 homology arms to support homology-directed repair at defined Fzd7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.