Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FOXJ1 Double Nickase Plasmid (h): sc-402226-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXJ1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXJ1 Double Nickase Plasmid (h) and FOXJ1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXJ1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXJ1 Antibody (3-19): sc-53139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXJ1 Double Nickase Plasmid (h) | sc-402226-NIC | 20 µg | $410.00 | |||
FOXJ1 Double Nickase Plasmid (h2) | sc-402226-NIC-2 | 20 µg | $410.00 |
FOXJ1 (forkhead box J1) encodes a forkhead family transcription factor that functions as a master regulator of motile ciliogenesis, coordinating basal body docking, axonemal assembly, and the differentiation of multiciliated epithelial cells. It controls transcriptional programs required for ciliary beating and mucociliary clearance, linking epithelial polarity and cytoskeletal organization to cilium-dependent signaling and fluid flow. FOXJ1 activity is central in airway and ependymal lineages, where precise regulation supports tissue homeostasis and developmental patterning. Dysregulated FOXJ1 expression or impaired FOXJ1-driven ciliogenesis is associated with human ciliopathy-related phenotypes, including defects in mucociliary function and hydrocephalus-relevant ependymal abnormalities.
FOXJ1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXJ1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXJ1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXJ1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXJ1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.