Fluorescein-dUTP (CAS 214154-36-6)
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Fluorescein-dUTP 1 mM in Tris Buffer (pH 7.5) is a compound used primarily in molecular biology research for the labeling of nucleic acids. The inclusion of fluorescein allows for the direct detection of the incorporated nucleotide via fluorescence microscopy or flow cytometry. This compound is instrumental in techniques such as fluorescence in situ hybridization (FISH), where it enables the visualization of specific DNA sequences within chromosomes. In addition, it is utilized in real-time PCR assays to monitor the amplification of target DNA sequences, as the fluorescein moiety provides a fluorescent signal that increases proportionally with the amount of DNA synthesized. The stabilization of Fluorescein-dUTP in Tris buffer at a neutral pH ensures that it is in an optimal condition for enzymatic reactions, which is critical for maintaining the fidelity and efficiency of nucleic acid synthesis during various molecular biology protocols.
Fluorescein-dUTP (CAS 214154-36-6) References
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fluorescein-dUTP, 25 nmol | sc-391125 | 25 nmol | $370.00 |