Date published: 2026-4-25

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Fluorescein-5-thiosemicarbazide Hydrochloride (CAS 76863-28-0)

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Alternate Names:
5 - FTSC
Application:
Fluorescein-5-thiosemicarbazide Hydrochloride is a compound used to label cell-surface functional groups
CAS Number:
76863-28-0
Molecular Weight:
457.89
Molecular Formula:
C21H15N3O5S*HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Fluorescein-5-thiosemicarbazide Hydrochloride is a water-soluble dye with fluorescent properties, widely utilized in various scientific research applications. As a derivative of fluorescein, it enables the detection and quantification of proteins, carbohydrates, and other biomolecules. Its applications extend to enzyme kinetics, immunological assays, and biochemical studies. This compound has proven effective in laboratory experiments, serving as useful for biomolecule detection and quantification. Moreover, Fluorescein-5-thiosemicarbazide Hydrochloride can be employed to detect and quantify DNA and RNA. Its mechanism of action involves the reaction between fluorescein and thiosemicarbazide, resulting in the production of a fluorescent product. This product is then utilized for the detection and quantification of proteins, carbohydrates, and other biomolecules. Fluorescein-5-thiosemicarbazide is used in labeling of cell-surface functional groups (glycophorins). It is a cell impermeant fluorescent probe useful for determining protein and peptide topology on the cell surface.


Fluorescein-5-thiosemicarbazide Hydrochloride (CAS 76863-28-0) References

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  3. Fluorescein-5-thiosemicarbazide as a probe for directly imaging of mucin-type O-linked glycoprotein within living cells.  |  Zhang, Y., et al. 2012. Glycoconj J. 29: 445-52. PMID: 22797904
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  5. An efficient method for selectively imaging and quantifying in situ the expression of sialylated glycoproteins on living cells.  |  Zhang, Y., et al. 2013. Glycobiology. 23: 643-53. PMID: 23172875
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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Fluorescein-5-thiosemicarbazide Hydrochloride, 50 mg

sc-211522
50 mg
$421.00