FLIP_S/L Antibody (G-11): sc-5276

FLIPS/L Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects FLIPS/L protein of mouse, rat, and human origin through western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. FLIPS/L monoclonal antibody (G-11) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. FLIP protein, which exists in both long and short forms, plays a crucial role in the regulation of apoptosis, a vital process for maintaining cellular homeostasis and preventing uncontrolled cell proliferation. The short form of FLIP features two death effector domains that are homologous to those found in the FAS-associating protein FADD, while the long form shares significant homology with the cysteine protease FLICE and includes an additional caspase-like domain. The long form lacks a catalytic active site and the necessary residues for substrate binding, which distinguishes FLIP from typical caspases. FLIP has been identified by various names, including Casper, I-FLICE, CLARP, FLAME-1, and MRIT, and while its precise function is still under investigation, FLIP is recognized as a key regulator of apoptosis downstream of all known death receptors, highlighting its importance in cellular signaling pathways and potential implications in cancer research and therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FLIP_S/L Antibody (G-11) References:

1. An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis.  |  Kim, Y., et al. 2002. J Biol Chem. 277: 22320-9. PMID: 11940602
2. FLIP protein and TRAIL-induced apoptosis.  |  Roth, W. and Reed, JC. 2004. Vitam Horm. 67: 189-206. PMID: 15110178
3. FLICE-like inhibitory protein (FLIP) protects against apoptosis and suppresses NF-kappaB activation induced by bacterial lipopolysaccharide.  |  Bannerman, DD., et al. 2004. Am J Pathol. 165: 1423-31. PMID: 15466406
4. Fever-like hyperthermia controls T Lymphocyte persistence by inducing degradation of cellular FLIPshort.  |  Meinander, A., et al. 2007. J Immunol. 178: 3944-53. PMID: 17339495
5. A chemical screen identifies anisomycin as an anoikis sensitizer that functions by decreasing FLIP protein synthesis.  |  Mawji, IA., et al. 2007. Cancer Res. 67: 8307-15. PMID: 17804746
6. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors.  |  Thome, M., et al. 1997. Nature. 386: 517-21. PMID: 9087414
7. Casper is a FADD- and caspase-related inducer of apoptosis.  |  Shu, HB., et al. 1997. Immunity. 6: 751-63. PMID: 9208847
8. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.  |  Hu, S., et al. 1997. J Biol Chem. 272: 17255-7. PMID: 9211860
9. Inhibition of death receptor signals by cellular FLIP.  |  Irmler, M., et al. 1997. Nature. 388: 190-5. PMID: 9217161
10. FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis.  |  Srinivasula, SM., et al. 1997. J Biol Chem. 272: 18542-5. PMID: 9228018
11. MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death.  |  Han, DK., et al. 1997. Proc Natl Acad Sci U S A. 94: 11333-8. PMID: 9326610
12. CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis.  |  Inohara, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 10717-22. PMID: 9380701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FLIP_S/L Antibody (G-11) has 118 citations in a variety of scientific publications.

Primary antibodies like Anti-FLIP_S/L Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FLIP_S/L Antibody (G-11) Specifications

Alternate Product Name: FLIP

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-202

Epitope Target: FLIP_S/L

Epitope Species: human

Product Reactivity/Detection: FLIP short and FLIP long

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 118 citations in various scientific publications

Available Conjugates/Forms for FLIP_S/L Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin