Ethylenediaminetetraacetic acid disodium salt solution (CAS 139-33-3)

Ethylenediaminetetraacetic acid disodium salt solution, commonly known as EDTA disodium, is a water-soluble form of EDTA, extensively used in biochemical research for its ability to chelate divalent metal ions. This chelating property is crucial in a variety of scientific studies, particularly in the preservation of biochemical and molecular integrity by inhibiting metal ion-dependent enzymatic processes. In research, EDTA disodium is used to sequester metal ions such as calcium and magnesium, which are essential cofactors for many enzymes, including DNases and RNases. By binding these ions, EDTA disodium prevents enzymatic activity that could otherwise degrade nucleic acids during extraction procedures, thereby ensuring the stability and purity of DNA and RNA samples. Furthermore, its application extends to analytical chemistry, where it is utilized in complexometric titrations to determine water hardness or to quantify the concentrations of metal ions in solutions. In cell biology, EDTA disodium is a key component in cell culture media and is used to facilitate the detachment of adherent cells by chelating calcium ions, disrupting cell-to-cell and cell-to-matrix interactions. This broad utility in binding metal ions not only underscores its importance in laboratory protocols but also contributes to its widespread use in studying metalloproteins and other metal-dependent biological phenomena.