Date published: 2025-9-5

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EPAS-1/HIF-2 alpha 抗体 (A-5): sc-46691

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说明书
  • EPAS-1/HIF-2 alpha抗体(A-5)是小鼠单克隆IgG2b κ, 在20篇文献中引用,规格为200 µg/ml
  • 免疫human物种的EPAS-1的氨基酸556-865
  • EPAS-1/HIF-2 alpha 抗体 (A-5) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 EPAS-1
  • 抗EPAS-1/HIF-2 alpha抗体(A-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-46691 X, 200 µg/0.1 ml)
  • 关于如何获取EPAS-1/HIF-2 alpha (A-5): sc-46691免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成EPAS-1/HIF-2 alpha 抗体 (A-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

EPAS-1抗体(A-5)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人类EPAS-1。EPAS-1 (A-5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。EPAS-1 也称为 HIF-2α,在细胞对缺氧(一种以低氧水平为特征的状态)的反应中起着至关重要的作用。EPAS-1主要位于细胞核中,作为转录因子调节血管生成、红细胞生成和葡萄糖代谢等关键过程的基因表达。EPAS-1能够与其他缺氧诱导因子(如HIF-1β (Arnt 1))形成异二聚体,从而与目标基因启动子中的缺氧响应元件结合,促进基因转录,帮助细胞适应缺氧环境。了解EPAS-1的功能和定位至关重要,因为EPAS-1失调与多种病理状况有关,包括癌症和心血管疾病,这些疾病通常与供氧不足有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

EPAS-1/HIF-2 alpha 抗体 (A-5)

sc-46691
200 µg/ml
$316.00

EPAS-1/HIF-2 alpha 抗体 (A-5) X

sc-46691 X
200 µg/0.1 ml
$316.00

EPAS-1/HIF-2 alpha 抗体 (A-5) AC

sc-46691 AC
500 µg/ml, 25% agarose
$416.00

EPAS-1/HIF-2 alpha 抗体 (A-5) HRP

sc-46691 HRP
200 µg/ml
$316.00

EPAS-1/HIF-2 alpha 抗体 (A-5) FITC

sc-46691 FITC
200 µg/ml
$330.00

EPAS-1/HIF-2 alpha 抗体 (A-5) PE

sc-46691 PE
200 µg/ml
$343.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 488

sc-46691 AF488
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 546

sc-46691 AF546
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 594

sc-46691 AF594
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 647

sc-46691 AF647
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 680

sc-46691 AF680
200 µg/ml
$357.00

EPAS-1/HIF-2 alpha 抗体 (A-5) Alexa Fluor® 790

sc-46691 AF790
200 µg/ml
$357.00

Can you provide the literature reference using the sc-46691 X for ChIP assays?

Asked by: Edwardfu11
Please contact Technical Service
Answered by: Tech Sales
Date published: 2021-05-12

Does this antibody recognize mouse/rat EPAS1? Can it be used for IHC? I want to find an EPAS1 Ab only recognizing human origin. Thanks, Xiaoyong

Asked by: Eddiefu
Thank you for your question. Currently we only recommend this mouse monoclonal antibody, EPAS-1 (A-5): sc-46691, for detection of human EPAS-1. However, we have not completed our testing of the antibody for possible cross-reactivity with other species, including mouse and rat. The antibody is raised against a recombinant protein that does share significant (78%) identity with the mouse EPAS-1 protein, so it is possible that it may cross-react with mouse.
Answered by: Technical Support
Date published: 2017-06-30

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 10
Date published: 2017-01-19
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Rated 4 out of 5 by from Resultado adecuadoEste anticuerpo detecta una única banda alrededor de 120KDa en las lineas celulares A549 y HCT116 cultivadas en condiciones de hipoxia durante 10h y 24h. A diferencia del anticuerpo ep190 que no reconoce nada en los mismos lisados.
Date published: 2018-04-17
Rated 5 out of 5 by from Great westernVery nice western blot data; strong signal with low background
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of EPAS-1 expression in untreated and CoCl2-treated HeLa whole cell lysates.
Date published: 2017-01-12
Rated 5 out of 5 by from Very strong signal with hypoxic human cancer cellsI have used this antibody for western blotting and obtained a very strong signal for hypoxic cancer cells, namely A549 and HT1080 cells
Date published: 2016-11-29
Rated 5 out of 5 by from Works in IHCWorks in IHC
Date published: 2015-03-19
Rated 5 out of 5 by from WB publishable data with human MCF 7WB publishable data with human MCF 7 - SCBT Publication Review
Date published: 2015-02-22
Rated 4 out of 5 by from Band seen in WB using CoCl2Band seen in WB using CoCl2-treated HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-02
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EPAS-1/HIF-2 alpha 抗体 (A-5) is rated 4.7 out of 5 by 7.
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