Eme1 Antibody (A-9): sc-393363
- Eme1 Antibody (A-9) is a mouse monoclonal IgG1, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 37-91 mapping near the N-terminus of Eme1 of mouse origin
- Anti-Eme1 Antibody (A-9) is recommended for detection of Eme1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Eme1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Eme1 Antibody (A-9) for WB applications. This reagent is now offered in a bundle with Eme1 Antibody (A-9) (see ordering information below).
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Eme1 Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects Eme1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Eme1 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Essential meiotic endonuclease 1 (Eme1) plays a critical role in DNA repair and recombination processes, particularly during meiosis and mitosis. Eme1 is a member of the Eme1/Mms4 family and forms a heterodimeric complex with MUS81, which is essential for cleaving branched DNA structures that can arise during DNA replication stress, such as stalled replication forks and 3′ DNA flaps. The MUS81-Eme1 complex resolves synthetic Holliday junctions into linear duplex DNA, highlighting Eme1′s importance in maintaining genomic stability. Eme1 primarily localizes to the nucleolus and is recruited to sites of DNA damage during the S phase of the cell cycle, underscoring Eme1′s vital role in the cellular response to DNA damage and the preservation of genetic integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Eme1 Antibody (A-9) References:
- Identification and characterization of the human mus81-eme1 endonuclease. | Ciccia, A., et al. 2003. J Biol Chem. 278: 25172-8. PMID: 12721304
- The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism. | Gaillard, PHL., et al. 2003. Mol Cell. 12: 747-59. PMID: 14527419
- The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks. | Hanada, K., et al. 2006. EMBO J. 25: 4921-32. PMID: 17036055
- Interplay between Np95 and Eme1 in the DNA damage response. | Mistry, H., et al. 2008. Biochem Biophys Res Commun. 375: 321-5. PMID: 18692478
- Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday junctions. | Geuting, V., et al. 2009. Plant Physiol. 150: 1062-71. PMID: 19339504
- Structure-specific DNA endonuclease Mus81/Eme1 generates DNA damage caused by Chk1 inactivation. | Forment, JV., et al. 2011. PLoS One. 6: e23517. PMID: 21858151
- Wee1 controls genomic stability during replication by regulating the Mus81-Eme1 endonuclease. | Domínguez-Kelly, R., et al. 2011. J Cell Biol. 194: 567-79. PMID: 21859861
- Regulation of Mus81-Eme1 Holliday junction resolvase in response to DNA damage. | Dehé, PM., et al. 2013. Nat Struct Mol Biol. 20: 598-603. PMID: 23584455
- Identification of small-molecule inhibitors of human MUS81-EME1/2 by FRET-based high-throughput screening. | Zhang, X., et al. 2023. Bioorg Med Chem. 90: 117383. PMID: 37352577
- SETD1A-dependent EME1 transcription drives PARPi sensitivity in HR deficient tumour cells. | Sweatman, E., et al. 2025. Br J Cancer.. PMID: 39994444
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eme1 Antibody (A-9) | sc-393363 | 200 µg/ml | $322.00 | |||
Eme1 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-531759 | 200 µg Ab; 20 µg BP | $361.00 | |||
Eme1 Antibody (A-9) AC | sc-393363 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Eme1 Antibody (A-9) HRP | sc-393363 HRP | 200 µg/ml | $322.00 | |||
Eme1 Antibody (A-9) FITC | sc-393363 FITC | 200 µg/ml | $336.00 | |||
Eme1 Antibody (A-9) PE | sc-393363 PE | 200 µg/ml | $349.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 488 | sc-393363 AF488 | 200 µg/ml | $364.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 546 | sc-393363 AF546 | 200 µg/ml | $364.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 594 | sc-393363 AF594 | 200 µg/ml | $364.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 647 | sc-393363 AF647 | 200 µg/ml | $364.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 680 | sc-393363 AF680 | 200 µg/ml | $364.00 | |||
Eme1 Antibody (A-9) Alexa Fluor® 790 | sc-393363 AF790 | 200 µg/ml | $364.00 |