Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m): sc-420081-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m) and Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dvl2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dvl-2/Dishevelled 2/DVL2 Antibody (D-6): sc-390303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m) | sc-420081-NIC | 20 µg | $410.00 |
Mouse Dvl2 encodes Dishevelled 2 (DVL2), a cytoplasmic scaffold that relays Wnt ligand signals from Frizzled receptors to downstream effectors. DVL2 integrates canonical Wnt/β-catenin transcriptional programs with non-canonical planar cell polarity and Wnt/Ca²⁺ pathways, influencing cell polarity, cytoskeletal remodeling, migration, and developmental patterning. Through modulation of pathway output, DVL2 contributes to context-dependent control of proliferation and differentiation, making it widely studied in tissue morphogenesis and stem cell biology. Dysregulated Wnt–Dishevelled signaling is implicated in congenital developmental defects and disease-associated signaling rewiring, supporting mechanistic investigations of pathway regulation in mouse models.
Dvl-2/Dishevelled 2/DVL2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dvl2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dvl2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dvl2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dvl2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.