DIAPH3 Antibody (4D5): sc-293288

DIAPH3 Antibody (4D5) is a mouse monoclonal IgG2a kappa light chain antibody that detects DIAPH3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). DIAPH3 (4D5) antibody is available as the non-conjugated form. DIAPH3, also known as diaphanous homolog 3, DRF3, or mDia2, is a crucial 1,193 amino acid member of the formin homology protein family, playing a vital role in various cellular processes, particularly in actin dynamics. Located primarily in the cytoplasm and at the cell membrane, DIAPH3 is essential for actin polymerization, which is critical for maintaining cell shape, motility, and division. DIAPH3 interacts with Profilin, a key regulator of actin filament assembly, and the GTP-bound form of Rho (Rho-GTP), facilitating the recruitment of Profilin to the membrane in a Rho-dependent manner. This interaction is pivotal for the formation of stress fibers and the successful completion of cytokinesis, as well as for the transcriptional activation of the serum response factor (SRF). DIAPH3 activity is tightly regulated through its diaphanous autoregulatory domain (DAD) and Rho GTPase-binding domain (GBD); when these domains are bound intramolecularly, DIAPH3 remains inactive. Disruption of this bond allows the GBD to engage with Rho-GTP, thus activating DIAPH3. Notably, alternative splicing of the DIAPH3 gene results in seven distinct isoforms, each potentially contributing to DIAPH3′s diverse functional roles in cellular signaling and structure.

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DIAPH3 Antibody (4D5) References:

1. Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42.  |  Peng, J., et al. 2003. Curr Biol. 13: 534-45. PMID: 12676083
2. Identification and characterization of human DIAPH3 gene in silico.  |  Katoh, M. and Katoh, M. 2004. Int J Mol Med. 13: 473-8. PMID: 14767582
3. Identification and characterization of human FHOD3 gene in silico.  |  Katoh, M. and Katoh, M. 2004. Int J Mol Med. 13: 615-20. PMID: 15010865
4. Identification and characterization of human FHDC1, mouse Fhdc1 and zebrafish fhdc1 genes in silico.  |  Katoh, M. and Katoh, M. 2004. Int J Mol Med. 13: 929-34. PMID: 15138637
5. Identification and characterization of CDC50A, CDC50B and CDC50C genes in silico.  |  Katoh, Y. and Katoh, M. 2004. Oncol Rep. 12: 939-43. PMID: 15375526
6. DIAPH3 promotes the tumorigenesis of lung adenocarcinoma.  |  Xiang, G., et al. 2019. Exp Cell Res. 385: 111662. PMID: 31586548
7. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.  |  Lau, EO., et al. 2021. Elife. 10: PMID: 33899739
8. Diaph3 underlines tumor cell heterogeneity in glioblastoma with implications for treatment modalities resistance.  |  Stoyanov, GS., et al. 2022. J Neurooncol. 157: 523-531. PMID: 35380294
9. DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling.  |  Zhang, K., et al. 2023. Cell Rep. 42: 111986. PMID: 36640348
10. Super Enhancer-Regulated LncRNA LINC01089 Induces Alternative Splicing of DIAPH3 to Drive Hepatocellular Carcinoma Metastasis.  |  Su, T., et al. 2023. Cancer Res. 83: 4080-4094. PMID: 37756562
11. DIAPH3 predicts survival of patients with MGMT-methylated glioblastoma.  |  Chehade, G., et al. 2024. Front Oncol. 14: 1359652. PMID: 38454929
12. The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow.  |  Shah, R., et al. 2024. Nat Commun. 15: 5250. PMID: 38897998

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DIAPH3 Antibody (4D5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DIAPH3 Antibody (4D5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DIAPH3 Antibody (4D5) Specifications

Alternate Product Name: DIAPH3

Product Clone Name: 4D5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 632-729

Epitope Target: DIAPH3

Epitope Species: human

Product Reactivity/Detection: DIAPH3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DIAPH3 Antibody (4D5): N/A