Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDB1 Double Nickase Plasmid (h): sc-402067-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDB1 Double Nickase Plasmid (h) and DDB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDB1 Antibody (E-11): sc-376860
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDB1 Double Nickase Plasmid (h) | sc-402067-NIC | 20 µg | $410.00 | |||
DDB1 Double Nickase Plasmid (h2) | sc-402067-NIC-2 | 20 µg | $410.00 |
DDB1 (damage specific DNA binding protein 1) is a core adaptor of the CRL4 (CUL4–RBX1) E3 ubiquitin ligase complex that recruits diverse substrate receptors to control ubiquitin-dependent protein turnover. In human cells, DDB1 coordinates genome maintenance pathways including nucleotide excision repair, replication stress responses, and checkpoint control by modulating stability of factors involved in DNA damage recognition and cell-cycle progression. Through these functions, DDB1 helps preserve chromatin integrity and transcriptional homeostasis under genotoxic stress. Dysregulation of DDB1-linked ubiquitination networks has been associated with altered DNA repair capacity and proliferative phenotypes relevant to studies of cancer biology and genome stability.
DDB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.