DcpS Double Nickase Plasmid (m): sc-427369-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DcpS Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DcpS Double Nickase Plasmid (m) and DcpS Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dcps. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DcpS Antibody (A-12): sc-393226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DcpS Double Nickase Plasmid (m) | sc-427369-NIC | 20 µg | $410.00 |
Mouse Dcps encodes the scavenger decapping enzyme DcpS, a cytosolic HIT-family hydrolase that cleaves residual cap structures (m7GpppN) generated during 3′→5′ mRNA decay. By hydrolyzing cap dinucleotides, DcpS supports mRNA turnover, nucleotide salvage, and prevents accumulation of cap derivatives that can perturb RNA metabolism. DcpS function connects to broader RNA surveillance and decay pathways, complementing cytoplasmic decapping and exosome-mediated processing. Dysregulated RNA processing and quality-control mechanisms are relevant to neurodevelopmental and neurodegenerative disease biology and to proliferative states where transcriptome homeostasis is remodeled.
DcpS Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dcps locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dcps. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dcps function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dcps-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.