DBH Antibody (DBH 41): sc-47707

DBH Antibody (DBH 41) is a mouse monoclonal IgG1 antibody that detects the DBH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-DBH antibody (DBH 41) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dopamine beta-hydroxylase (DBH) plays a crucial role in the biosynthesis of catecholamines by catalyzing the conversion of dopamine to noradrenaline, which is essential for the proper functioning of the sympathetic nervous system and the regulation of blood pressure. DBH is predominantly localized in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, making DBH a specific marker for noradrenergic processes. The active form of DBH is a homotetramer, found in the lumen of synaptic vesicles, where DBH is strategically positioned to facilitate the rapid conversion of neurotransmitters. Additionally, DBH expression is influenced by nerve growth factor and insulin growth factor-1, and DBH is regulated by intracellular second messengers such as protein kinase A, cyclic AMP, diacylglycerol, and calcium ions. Transcriptional regulation of DBH is mediated by key proteins including Sp1, CREB, and AP-1 family members like c-Fos, c-Jun, and JunD, highlighting DBH′s complex regulatory mechanisms and importance in neurobiology.

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DBH Antibody (DBH 41) References:

1. Rat dopamine beta-hydroxylase: molecular cloning and characterization of the cDNA and regulation of the mRNA by reserpine.  |  McMahon, A., et al. 1990. J Neurosci Res. 25: 395-404. PMID: 2325165
2. Human dopamine beta-hydroxylase gene: two mRNA types having different 3'-terminal regions are produced through alternative polyadenylation.  |  Kobayashi, K., et al. 1989. Nucleic Acids Res. 17: 1089-102. PMID: 2922261
3. The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme.  |  Lamouroux, A., et al. 1987. EMBO J. 6: 3931-7. PMID: 3443096
4. Induction of gene expression of the catecholamine-synthesizing enzymes by insulin-like growth factor-I.  |  Hwang, O. and Choi, HJ. 1995. J Neurochem. 65: 1988-96. PMID: 7595482
5. AP1 proteins mediate the cAMP response of the dopamine beta-hydroxylase gene.  |  Swanson, DJ., et al. 1998. J Biol Chem. 273: 24065-74. PMID: 9727025
6. Noradrenergic-specific transcription of the dopamine beta-hydroxylase gene requires synergy of multiple cis-acting elements including at least two Phox2a-binding sites.  |  Kim, HS., et al. 1998. J Neurosci. 18: 8247-60. PMID: 9763470

The preferred DBH antibody is DBH Antibody (A-9): sc-365710.