CYP2E1 Double Nickase Plasmid (h): sc-401357-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP2E1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYP2E1 Double Nickase Plasmid (h) and CYP2E1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYP2E1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP2E1 Double Nickase Plasmid (h) | sc-401357-NIC | 20 µg | $410.00 | |||
CYP2E1 Double Nickase Plasmid (h2) | sc-401357-NIC-2 | 20 µg | $410.00 |
Cytochrome P450 2E1 (CYP2E1) is a microsomal monooxygenase that oxidizes small organic substrates and contributes to phase I xenobiotic metabolism in the endoplasmic reticulum. It participates in redox cycling and can generate reactive oxygen species, linking CYP2E1 activity to oxidative stress, lipid peroxidation, and downstream inflammatory signaling. CYP2E1 intersects with hepatic detoxification pathways and influences cellular responses to alcohol and chemical exposures through metabolism of multiple low–molecular weight compounds. Altered CYP2E1 expression or activity has been associated with susceptibility to toxicant-induced liver injury and metabolic stress phenotypes, making it a useful target for mechanistic studies of xenobiotic processing.
CYP2E1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYP2E1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYP2E1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYP2E1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYP2E1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.