Molecular structure of Chymotrypsin Substrate II, Fluorogenic, CAS Number: 88467-45-2
Chymotrypsin Substrate II, Fluorogenic (CAS 88467-45-2)
Alternate Names:
N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin; Suc-AAPF-AMC
Application:
Chymotrypsin Substrate II, Fluorogenic is a quantitative fluorogenic chymotrypsin substrate
CAS Number:
88467-45-2
Purity:
≥98%
Molecular Weight:
661.70
Molecular Formula:
C34H39N5O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
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SDS & Certificate of Analysis
Chymotrypsin Substrate II, Fluorogenic is a specially designed peptide that serves as a substrate for the enzyme chymotrypsin, a digestive protease that cleaves peptide bonds at the carboxyl side of aromatic amino acids like tyrosine, tryptophan, and phenylalanine. The fluorogenic aspect of this substrate means that upon enzymatic cleavage by chymotrypsin, it releases a fluorescent signal, allowing for real-time monitoring of the enzyme′s activity. This property makes it an invaluable tool in various research and analytical applications, particularly in the study of protease activity and inhibitor screening. By measuring the increase in fluorescence intensity as the substrate is cleaved, researchers can determine the enzyme′s velocity, substrate affinity (Km), and catalytic efficiency (kcat), providing insights into its catalytic mechanism.
Chymotrypsin Substrate II, Fluorogenic (CAS 88467-45-2) References
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- Synergistic Activity between Two Antifungal Proteins, the Plant Defensin NaD1 and the Bovine Pancreatic Trypsin Inhibitor. | Bleackley, MR., et al. 2017. mSphere. 2: PMID: 29062897
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- Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation. | Burgener, SS., et al. 2019. Cell Rep. 27: 3646-3656.e5. PMID: 31216481
- Screening and Identification of Metacaspase Inhibitors: Evaluation of Inhibition Mechanism and Trypanocidal Activity. | Pérez, B., et al. 2021. Antimicrob Agents Chemother. 65: PMID: 33318019
- Reversible Photoswitchable Inhibitors Generate Ultrasensitivity in Out-of-Equilibrium Enzymatic Reactions. | Teders, M., et al. 2021. J Am Chem Soc. 143: 5709-5716. PMID: 33844531
- Proteolytic Cleavage of Bioactive Peptides and Protease-Activated Receptors in Acute and Post-Colitis. | De Bruyn, M., et al. 2021. Int J Mol Sci. 22: PMID: 34639054
- The Effect of Serine Protease Inhibitors on Visceral Pain in Different Rodent Models With an Intestinal Insult. | Ceuleers, H., et al. 2022. Front Pharmacol. 13: 765744. PMID: 35721192
- Extracellular Enzymatic Activities of Oceanic Pelagic Fungal Strains and the Influence of Temperature. | Salazar Alekseyeva, K., et al. 2022. J Fungi (Basel). 8: PMID: 35736054
- Discovery and molecular basis of subtype-selective cyclophilin inhibitors. | Peterson, AA., et al. 2022. Nat Chem Biol. 18: 1184-1195. PMID: 36163383
Substrate of:
Fluorogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Chymotrypsin Substrate II, Fluorogenic, 25 mg | sc-391097 | 25 mg | $189.00 |