Cefamandole sodium salt (CAS 30034-03-8)

Cefamandole sodium salt, listed under CAS number 30034-03-8, is a sodium salt form of cefamandole, a second-generation cephalosporin antibiotic that has been extensively utilized in scientific research to investigate bacterial cell wall synthesis and resistance mechanisms. The primary mode of action of cefamandole involves the inhibition of bacterial cell wall biosynthesis. This is achieved through its binding to specific penicillin-binding proteins (PBPs) located on the inner membrane of the bacterial cell wall. These PBPs are critical enzymes involved in the final stages of assembling the peptidoglycan matrix, which provides structural strength to the bacterial cell wall. By binding to these proteins, cefamandole disrupts their normal function, thereby inhibiting the cross-linking of peptidoglycan chains which is essential for bacterial cell wall integrity and rigidity. As a result, the process leads to cell wall weakening, culminating in cell lysis and bacterial death. In research, cefamandole sodium salt is particularly valuable for studying the kinetics and affinity of cephalosporin interactions with various PBPs across different bacterial species. It also helps in understanding the genetic basis of bacterial resistance to cephalosporins, especially concerning the modification of PBPs and the production of beta-lactamase enzymes that degrade cephalosporin molecules. These studies are crucial for advancing knowledge on bacterial resistance patterns and for the development of novel strategies to combat bacterial infections.