Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD24 Double Nickase Plasmid (h): sc-418762-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD24 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD24 Double Nickase Plasmid (h) and CD24 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD24. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD24 Antibody (SN3): sc-19585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD24 Double Nickase Plasmid (h) | sc-418762-NIC | 20 µg | $410.00 | |||
CD24 Double Nickase Plasmid (h2) | sc-418762-NIC-2 | 20 µg | $410.00 |
CD24 encodes a heavily glycosylated, GPI-anchored cell-surface protein that functions as a modulator of cell–cell and cell–matrix interactions and helps tune immune and inflammatory signaling. CD24 can regulate B-cell maturation and antigen-responsive pathways, and it participates in inhibitory signaling through interactions with Siglec receptors, shaping innate immune activation and cytokine output. In epithelial and hematopoietic contexts, altered CD24 expression is associated with changes in adhesion, migration, and differentiation programs, linking it to processes such as metastasis-like behavior and tumor microenvironment crosstalk in multiple cancer models. CD24 is also used as a lineage and stem/progenitor-associated marker in diverse tissues, supporting mechanistic studies of cellular heterogeneity and state transitions.
CD24 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD24 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD24. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD24 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD24-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.