Date published: 2025-9-5
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CD15 Antibody (C3D-1) - Immunohistochemistry - Image 156539
CD15 抗体 (C3D-1): sc-19648. 福尔马林固定, 石蜡包埋的人脾组织的免疫过氧化物酶染色显示红髓细胞亚群的胞浆染色.
CD15 抗体 (C3D-1): sc-19648. 福尔马林固定, 石蜡包埋的人脾组织的免疫过氧化物酶染色显示红髓细胞亚群的胞浆染色.

CD15 抗体 (C3D-1): sc-19648

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说明书
  • CD15 抗体 C3D-1 是小鼠单克隆 IgM κ，CD15抗体, 在11篇文献中引用，规格为200 µg/ml
  • 针对纯化的human来源中性粒细胞
  • 推荐用于 mouse, rat 和 human 来源的CD15 WB, IP, IF, IHC(P) 和 FCM检测
  • 可与荧光素（sc-19648 FITC）或Alexa Fluor® 488 （sc-19648 AF488）、200 µg/ml，用于IF、IHC(P)和FCM，
  • 关于如何获取CD15 (C3D-1): sc-19648的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD15 抗体 (C3D-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD15 抗体包括 CD15s

CD15抗体（C3D-1）是一种小鼠单克隆IgM抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的CD15蛋白。CD15（C3D-1）抗体有非结合型和多种结合型，包括藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合型。CD15，又称Lewis X或LeX，是由Fucosyltransferase IV（FucT-IV）合成的碳水化合物抗原，该酶催化将岩藻糖添加到糖受体分子上，形成对细胞表面相互作用至关重要的三糖。CD15主要位于白细胞和某些肿瘤细胞的表面，作为粘附分子，CD15在钙介导的同型结合中发挥着至关重要的作用。CD15在细胞表面的存在促进了细胞之间的强烈自聚，增强了它们在钙离子存在下相互作用的的能力。CD15是选择素（selectins）的配体，而选择素对于介导白细胞特异性细胞相互作用至关重要，因此CD15在免疫反应和炎症中的重要性不言而喻。CD15能够介导这些相互作用，因此成为免疫学和癌症生物学研究的重要目标，因为了解CD15的功能有助于深入了解细胞粘附过程和潜在的治疗干预措施。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD15 抗体 (C3D-1) 参考文献:

  1. 路易斯（x）糖决定因子在角膜上皮细胞粘附和分化中的作用。  |  Cao, Z., et al. 2001. J Biol Chem. 276: 21714-23. PMID: 11278542
  2. 作为粘附分子的 Le(X) 和相关结构。  |  Hakomori, S. 1992. Histochem J. 24: 771-6. PMID: 1362193
  3. 缺乏α1,3-岩藻糖基转移酶 IX 的小鼠表现出大脑中路易斯 x 结构的消失和焦虑样行为的增加。  |  Kudo, T., et al. 2007. Glycobiology. 17: 1-9. PMID: 16973732
  4. 单核细胞与黏合剂和病原体受体 DC-SIGN 的结合受 Lewis X 分子密度的调节。  |  Gijzen, K., et al. 2007. Mol Immunol. 44: 2481-6. PMID: 17175026
  5. 具有抗癌药物耐药性的人类卵巢癌衍生物细胞中路易斯抗原糖脂的特征性表达。  |  Iwamori, M., et al. 2007. J Biochem. 141: 309-17. PMID: 17190787
  6. Lex 与 Lex 决定因子之间的特异性相互作用。植入前胚胎和胚胎癌细胞中细胞识别的可能基础。  |  Eggens, I., et al. 1989. J Biol Chem. 264: 9476-84. PMID: 2470757
  7. CD15 免疫染色可改善胎盘对胎儿缺氧的诊断。  |  Seidmann, L., et al. 2021. Placenta. 105: 41-49. PMID: 33545630
  8. CD15+ 骨髓源性细胞是产生 ARG1 的结直肠癌细胞免疫反应的调节因子  |  Miyoshi, K., et al. 2022. Anticancer Res. 42: 459-470. PMID: 34969756
  9. SSEA-1与乳腺癌的侵袭性表型相关。  |  Szlasa, W., et al. 2022. Cancers (Basel). 14: PMID: 35565333
  10. 表达 CD30 和 CD15 的外周 T 细胞淋巴瘤扩大了 ALK 阴性无性大细胞淋巴瘤的范围。  |  Ganapathi, KA., et al. 2024. Br J Haematol. 204: 1862-1871. PMID: 38613165
  11. 髓系细胞上与 CD15（Lewis x）相关的碳水化合物结构是人类 CD2 的新型配体。  |  Warren, HS., et al. 1996. J Immunol. 156: 2866-73. PMID: 8609406
  12. CD34 选择和补体介导的免疫清除（抗 CD15 单克隆抗体）相结合，可以清除肿瘤细胞，同时保留正常的祖细胞。  |  Nimgaonkar, M., et al. 1996. J Hematother. 5: 39-48. PMID: 8646480

订购信息

产品名称产品编号规格价格数量收藏夹

CD15 抗体 (C3D-1)

sc-19648
200 µg/ml
$316.00

CD15 抗体 (C3D-1) FITC

sc-19648 FITC
200 µg/ml
$330.00

CD15 抗体 (C3D-1) Alexa Fluor® 488

sc-19648 AF488
200 µg/ml
$357.00

Who is the host? Mouse or the rabbit?

Asked by: wojtekszlasa
The host is mouse.
Answered by: Technical Service
Date published: 2022-05-26

Is there any information available on the specificity of sc-19648 (i.e., binding affinity for Lewis-X in terminal position on glycan chain, Lewis-X in internal position, etc.)?

Asked by: broccoli
Thank you for your question. This antibody was raised against purified neutrophils of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03

Does this antibody require any antigen retrieval procedure during paraffin IHC? 

Asked by: Masha
Thank you for your question. Yes, we recommend using 10 mM sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this mouse monoclonal IgM, CD15 Antibody (C3D-1): sc-19648. A link to our complete recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-21

How can CD15 (C3D-1): sc-19648 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, CD15 (C3D-1), is currently available conjugated to phycoerythrin (sc-19648 PE), fluorescein (sc-19648 FITC), Alexa Fluor® 488 (sc-19648 AF488), Alexa Fluor® 647 (sc-19648 AF647), PerCP (sc-19648 PerCP)
Answered by: Technical Service 11
Date published: 2017-01-17

What negative control should be used when using the conjugated version of CD15 (C3D-1): sc-19648 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-19648 PE, then you would want to use normal mouse IgGM PE, sc-2870.
Answered by: Technical Support 5
Date published: 2017-01-16
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Rated 4 out of 5 by from Nice immunohistochemistryI tried this antibody on mouse colon (FFPE) with a 1:50 dilution and an antigen retrieval heat-induced (Tris-EDTA pH 9.0). Immunoreactivity was nice and specific. Tried the sample also on canine species, but, unfortunately, it does not cross-react.
Date published: 2021-02-12
Rated 5 out of 5 by from excellent antibodyI use the pig colon cryosections to do immunofluorescence stain with CD15 sc19648. I got perfect images result.
Date published: 2017-08-18
Rated 5 out of 5 by from specific bandShows good staining strength in IHC with paraffin-embedded sections.
Date published: 2017-08-15
Rated 5 out of 5 by from Outstanding !!Great product for IHC staining; also strong IF and western blot results
Date published: 2017-01-24
Rated 5 out of 5 by from Highly recommended for IHC(P) and WB.This CD15 antibody is well characterized for use in WB, IF, FCM and IHC(P) on human and mouse samples.
Date published: 2017-01-17
Rated 4 out of 5 by from Publishable data in ICC using human brain tumorPublishable data in ICC using human brain tumor -SCBT Publication Review
Date published: 2015-07-16
Rated 4 out of 5 by from Publishable data in FCM using mouse blood(Publishable data in FCM using mouse blood( hepatic portal vein ) -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from Got strong results in IHCGot strong results in IHC
Date published: 2015-04-15
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CD15 抗体 (C3D-1) is rated 4.6 out of 5 by 11.
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