CD137 Double Nickase Plasmid (h): sc-405068-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD137 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD137 Double Nickase Plasmid (h) and CD137 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFRSF9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD137 Antibody (BBK-2): sc-58947
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD137 Double Nickase Plasmid (h) | sc-405068-NIC | 20 µg | $410.00 | |||
CD137 Double Nickase Plasmid (h2) | sc-405068-NIC-2 | 20 µg | $410.00 |
TNFRSF9 (CD137, 4-1BB) is an inducible TNF receptor superfamily member expressed on activated T cells, NK cells, and other immune subsets, where it functions as a co-stimulatory checkpoint controlling activation, survival, and effector differentiation. Engagement of CD137 by its ligand triggers adaptor recruitment and downstream NF-κB, MAPK, and PI3K/AKT signaling, supporting cytokine production, proliferation, and metabolic fitness. This pathway shapes immune cell–immune cell interactions within inflamed tissues and the tumor microenvironment and is frequently studied in the context of chronic inflammation, autoimmunity, and cancer immunobiology. Altered TNFRSF9 expression or signaling dynamics can modulate cytotoxic responses and immune exhaustion states, making it a useful node for mechanistic studies of immune regulation.
CD137 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFRSF9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFRSF9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFRSF9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFRSF9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.