CatSper CRISPR Activation Plasmid (h): sc-403796-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CatSper CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- CatSper CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by CatSper CRISPR Activation Plasmid (h) and CatSper CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CATSPER1 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CatSper CRISPR Activation Plasmid (h) | sc-403796-ACT | 20 µg | $397.00 |
CATSPER1 encodes CatSper, a sperm-specific, pH- and voltage-activated calcium channel subunit that contributes to the CatSper complex in the flagellar membrane. CatSper-mediated Ca2+ influx is a central regulator of sperm hyperactivated motility and capacitation-associated signaling, integrating ion homeostasis with downstream changes in flagellar beating and energy utilization. Disruption of CATSPER1 function perturbs calcium-dependent motility programs and is linked to impaired male fertility phenotypes, making it a key target for mechanistic studies of sperm physiology. In human reproductive biology research, CATSPER1 is also used to interrogate how ion channel regulation interfaces with second-messenger pathways and sperm function under physiological and stress conditions.
CatSper CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CATSPER1 expression without altering the underlying DNA sequence.
CatSper CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CATSPER1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CATSPER1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous CatSper expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CATSPER1 locus and enabling the study of CatSper-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of CatSper pathway restoration in tumor cells with silenced or reduced CATSPER1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.