Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKKβ Double Nickase Plasmid (h): sc-400928-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKKβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CaMKKβ Double Nickase Plasmid (h) and CaMKKβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMKK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKKβ Antibody (C-11): sc-271674
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKKβ Double Nickase Plasmid (h) | sc-400928-NIC | 20 µg | $410.00 | |||
CaMKKβ Double Nickase Plasmid (h2) | sc-400928-NIC-2 | 20 µg | $410.00 |
CAMKK2 encodes calcium/calmodulin-dependent protein kinase kinase 2 (CaMKKβ), an upstream Ser/Thr kinase that links Ca2+–calmodulin signaling to phosphorylation of AMPK and CaMKI/CaMKIV, thereby coordinating cellular energy sensing, transcriptional programs, and calcium-dependent stress responses. Through these nodes, CaMKKβ influences metabolic remodeling, mitochondrial function, autophagy, and downstream pathways such as mTOR and CREB-associated signaling. CAMKK2 activity has been studied in contexts including cancer cell metabolism, inflammation and immune cell polarization, and neuronal signaling, making it a useful target for dissecting calcium-driven kinase cascades. Perturbation of CAMKK2 can alter proliferative and survival phenotypes and has been associated with disease-relevant changes in metabolic and neuroendocrine regulation in experimental systems.
CaMKKβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAMKK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAMKK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAMKK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAMKK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.