Calponin 1/3 Antibody (E-4): sc-136986

Calponin 1/3 Antibody (E-4) is a mouse monoclonal IgG2a antibody that detects Calponin in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Calponin plays a crucial role in regulating smooth muscle contraction and serves as a marker for smooth muscle cell differentiation, making Calponin essential for understanding vascular and respiratory functions. Calponin is primarily located in the cytoplasm of smooth muscle cells, where Calponin interacts with actin and tropomyosin to modulate contractile activity. This localization allows Calponin to effectively inhibit actomyosin interactions, thereby controlling muscle contraction dynamics. The three isoforms—Calponin 1, Calponin 2, and Calponin 3—exhibit distinct expression patterns, with Calponin 2 also found in heart muscle and Calponin 3 in the brain, highlighting diverse physiological roles across different tissues. Calponin functionality is significantly influenced by post-translational modifications, particularly phosphorylation at Serine 175, which can alter inhibitory effects on smooth muscle contraction. Anti-Calponin antibody (E-4) is an invaluable tool for researchers investigating smooth muscle biology and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Calponin 1/3 Antibody (E-4) References:

1. Identification of calponin 3 as a novel Smad-binding modulator of BMP signaling expressed in cartilage.  |  Haag, J. and Aigner, T. 2007. Exp Cell Res. 313: 3386-94. PMID: 17825283
2. Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1.  |  Kurpinski, K., et al. 2009. Cell Mol Bioeng. 2: 606-614. PMID: 20037637
3. A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for Early B Cell Development.  |  Flemming, A., et al. 2015. PLoS One. 10: e0128385. PMID: 26046660
4. Calponin isoforms CNN1, CNN2 and CNN3: Regulators for actin cytoskeleton functions in smooth muscle and non-muscle cells.  |  Liu, R. and Jin, JP. 2016. Gene. 585: 143-153. PMID: 26970176
5. Fully automatic segmentation of type B aortic dissection from CTA images enabled by deep learning.  |  Cao, L., et al. 2019. Eur J Radiol. 121: 108713. PMID: 31683252
6. [12]aneN3-modified camptothecin and PEGylated AIEgens co-assembly into core-shell nanoparticles with ROS/NTR dual-response for enhanced cancer therapy.  |  Sun, XY., et al. 2023. J Mater Chem B. 11: 8943-8955. PMID: 37727888
7. Immune response and mesenchymal transition of papillary thyroid carcinoma reflected in ultrasonography features assessed by radiologists and deep learning.  |  Lee, J., et al. 2024. J Adv Res. 62: 219-228. PMID: 37783270
8. A deep learning model for brain age prediction using minimally preprocessed T1w images as input.  |  Dartora, C., et al. 2023. Front Aging Neurosci. 15: 1303036. PMID: 38259636
9. A deep learning approach to identify the fetal head position using transperineal ultrasound during labor.  |  Ramirez Zegarra, R., et al. 2024. Eur J Obstet Gynecol Reprod Biol. 301: 147-153. PMID: 39137593
10. Enhanced skin cancer diagnosis through grid search algorithm-optimized deep learning models for skin lesion analysis.  |  Pillai, R., et al. 2024. Front Med (Lausanne). 11: 1436470. PMID: 39574908

The preferred Calponin 1 antibody is Calponin 1 Antibody (CALP): sc-58707.