Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Caldesmon Double Nickase Plasmid (h): sc-402040-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Caldesmon Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Caldesmon Double Nickase Plasmid (h) and Caldesmon Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CALD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Caldesmon Antibody (C21): sc-58700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Caldesmon Double Nickase Plasmid (h) | sc-402040-NIC | 20 µg | $410.00 | |||
Caldesmon Double Nickase Plasmid (h2) | sc-402040-NIC-2 | 20 µg | $410.00 |
CALD1 encodes caldesmon, an actin- and calmodulin-binding protein that modulates actomyosin interactions and stabilizes cytoskeletal architecture in smooth muscle and non-muscle cells. By regulating actin filament dynamics and myosin ATPase activity in a calcium-dependent manner, caldesmon coordinates stress fiber organization, focal adhesion turnover, and contractile responses that influence cell shape and motility. CALD1 function intersects with Rho GTPase–driven cytoskeletal remodeling and signaling pathways that couple mechanical tension to transcriptional programs. Altered caldesmon expression or isoform balance has been associated with changes in cell migration and invasion phenotypes in cancer biology and with dysregulated smooth muscle behavior relevant to vascular remodeling and fibroproliferative processes.
Caldesmon Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CALD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CALD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CALD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CALD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.