Calcein-AM CAS: 148504-34-1
MF: C46H46N2O23
MW: 994.86
A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule.

Calcein-AM (CAS 148504-34-1)

Calcein-AM | CAS 148504-34-1 is rated 5.0 out of 5 by 3.
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Alternate Names: Calcein-AM also known as N,N′-[[3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1′-Bis[(acetyloxy)methyl] Ester; Calcein Acetoxymethyl Ester; NSC 689290
Application: Calcein-AM is a non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule
CAS Number: 148504-34-1
Molecular Weight: 994.86
Molecular Formula: C46H46N2O23
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to calcein (sc-202090). Calcein-AM is an extremely fluorescent and negatively charged molecule. The Calcein-AM probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein-AM as a cytosolic fluorophore. Calcein-AM has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.


References

1. Feller, N., et al. 1995. Br. J. Cancer. 72: 543-549. PMID: 7669559
2. Feller, N., et al. 1995. FEBS Lett. 368: 385-388. PMID: 7628644
3. Essodaigui, M., et al. 1998. Biochemistry. 37: 2243-2250. PMID: 9485370
4. Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta. 1366: 177-196. PMID: 9714796
5. Dhar, S., et al. 1998. Eur. J. Pharmacol. 346: 315-322. PMID: 9652375

Physical State :
Solid
Solubility :
Soluble in DMSO (10 mg/ml).
Storage :
Desiccate at -20° C
Boiling Point :
~982.73° C at 760 mmHg (Predicted)
Density :
~1.49 g/cm3 (Predicted)
Refractive Index :
n20D 1.61 (Predicted)
Fluoresence :
λex 496 nm, λem 516 nm in 0.1 M Tris pH 8.0
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
390986
MDL Number :
MFCD05861516
SMILES :
CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C

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Certificate of Analysis

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Calcein-AM (CAS 148504-34-1) Product Citations

See how others have used Calcein-AM (CAS 148504-34-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 46 total

PMID: # 31146416  Bociaga, D.|Sobczyk-Guzenda, A.|Komorowski, P.|Balcerzak, J.|Jastrzebski, K.|Przybyszewska, K.|Kaczmarek, A.| et al. 2019. Nanomaterials (Basel). 9:

PMID: # 30315883  Zhang, L.|Xu, Y.|Wang, L.|Liu, H.| et al. 2019. Int. J. Biol. Macromol. 121: 1077-1085.

PMID: # 30790546  Thege, FI.|Gruber, CN.|Cardle, II.|Cong, SH.|Lannin, TB.|Kirby, BJ.| et al. 2019. Anal. Biochem.

PMID: # 31102787  2019. Biochim Biophys Acta Mol Basis Dis. 1865: 2303-2316.

PMID: # 29799304  2019. Platelets. 30: 487-497.

PMID: # 31004314  Jiang, DQ.|Ma, YJ.|Wang, Y.|Lu, HX.|Mao, SH.|Zhao, SH.| et al. 2019. J Neural Transm (Vienna). 126: 559-568.

PMID: # 31315252  Mambetsariev, I.|Mirzapoiazova, T.|Lennon, F.|Jolly, MK.|Li, H.|Nasser, MW.|Vora, L.|Kulkarni, P.|Batra, SK.|Salgia, R.| et al. 2019. J Clin Med. 8:

PMID: # 29653579  Tong, WY. et al. 2018. J Nanobiotechnology. 16: 38.

PMID: # 29684377  Tocchetti, GN. et al. 2018. Biochem. Pharmacol. 154: 118-126.

PMID: # 29537109  Delve, E.|Parreno, J.|Co, V.|Wu, PH.|Chong, J.|Di Scipio, M.|Kandel, RA.| et al. 2018. J. Orthop. Res. 36: 2421-2430.

Citations 1 to 10 of 46 total

Is this product synthetic or of animal origin? Can it be considered as animal free?

Asked by: two2igm05
This product is synthetic and animal-free.
Answered by: Chemical Support 4
Date published: 2017-02-25

How should the stock solution in DMSO be stored?

Asked by: chemicalsmg
Thank you for your question. We recommend to aliquot and store the DMSO stock solution at -20ºC for this chemical, Calcein-AM (CAS 148504-34-1): sc-203865.
Answered by: Technical Support
Date published: 2016-12-13
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Rated 5 out of 5 by from Work very well Using this product for cytotoxicity assay, it work very well
Date published: 2020-03-06
Rated 5 out of 5 by from works well depending on your application staining requires some optimization
Date published: 2017-12-01
Rated 5 out of 5 by from Pandey Pandey, MK. et al. (PubMed 17673602) used Calcein-AM, a nonfluorescent polyanionic dye that is retained by live cells and which produces intense green fluorescence through enzymatic (esterase) conversion, to measure apoptosis. -SCBT Publication Review
Date published: 2015-01-23
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