Molecular structure of Calcein, CAS Number: 1461-15-0
Calcein (CAS 1461-15-0)
See product citations (7)
Alternate Names:
Fluorescein complex; Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein; Fluorescein-bis(methyliminodiacetic acid)
Application:
Calcein is a compound used for fluorometric determination of calcium and EDTA titration
CAS Number:
1461-15-0
Purity:
≥90%
Molecular Weight:
622.55
Molecular Formula:
C30H26N2O13
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Calcein is used for fluorometric determination of calcium and EDTA titration in the presence of magnesium. Calcein AM, the acetomethoxy ester of calcein, can be transported into living cells through the cellular membrane. Once in the cell, the acetomethoxy group is removed by intracellular esterases and the molecule trapped inside fluoresces green. It is also used to detect drug interactions with multidrug resistance proteins because it is an effective substrate of the multidrug resistance transporter 1P-glycoprotein and the multidrug resistance-related protein.
Calcein (CAS 1461-15-0) References
- Calcein as a fluorescent probe for ferric iron. Application to iron nutrition in plant cells. | Thomas, F., et al. 1999. J Biol Chem. 274: 13375-83. PMID: 10224100
- Calcein permeability of liposomes mediated by type A botulinum neurotoxin and its light and heavy chains. | Fu, FN. and Singh, BR. 1999. J Protein Chem. 18: 701-7. PMID: 10609647
- Calcein assay for multidrug resistance reliably predicts therapy response and survival rate in acute myeloid leukaemia. | Karászi, E., et al. 2001. Br J Haematol. 112: 308-14. PMID: 11167823
- Calcein as a fluorescent iron chemosensor for the determination of low molecular weight iron in biological fluids. | Ali, A., et al. 2003. Biometals. 16: 285-93. PMID: 12572687
- The synergic effects of various electrolytes and electroporation on the in vitro skin permeation of calcein. | Tokudome, Y. and Sugibayashi, K. 2003. J Control Release. 92: 93-101. PMID: 14499188
- The role of ABC transporters in drug resistance, metabolism and toxicity. | Glavinas, H., et al. 2004. Curr Drug Deliv. 1: 27-42. PMID: 16305368
- Simultaneous measurements of K+ and calcein release from liposomes and the determination of pore size formed in a membrane. | Katsu, T., et al. 2007. Anal Sci. 23: 517-22. PMID: 17495394
- Determination of ketotifen by using calcein as chemiluminescence reagent. | Fei, N. and Jiuru, L. 2007. Anal Chim Acta. 592: 168-72. PMID: 17512822
- Nano-sized water-in-oil-in-water emulsion enhances intestinal absorption of calcein, a high solubility and low permeability compound. | Koga, K., et al. 2010. Eur J Pharm Biopharm. 74: 223-32. PMID: 19755156
- Detection of volume changes in calcein-stained cells using confocal microscopy. | Davidson, AF. and Higgins, AZ. 2013. J Fluoresc. 23: 393-8. PMID: 23468245
- Investigation of Microbubble Cavitation-Induced Calcein Release from Cells In Vitro. | Maciulevičius, M., et al. 2016. Ultrasound Med Biol. 42: 2990-3000. PMID: 27637933
- Calcein leakage as a robust assay for cytochrome c/H2O2-mediated liposome permeabilization. | Firsov, AM., et al. 2018. Anal Biochem. 552: 19-23. PMID: 28322801
- Contribution of headgroup and chain length of glycerophospholipids to thermal stability and permeability of liposomes loaded with calcein. | Prislan, I., et al. 2019. Chem Phys Lipids. 225: 104807. PMID: 31390525
- Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells. | Kitchen, P., et al. 2020. STAR Protoc. 1: 100157. PMID: 33377051
- Cytoplasmic delivery of calcein mediated by liposomes modified with a pH-sensitive poly(ethylene glycol) derivative. | Kono, K., et al. 1997. Biochim Biophys Acta. 1325: 143-54. PMID: 9168140
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calcein, 1 g | sc-202090 | 1 g | $45.00 |