Bu-1a/b Antibody (AV20): sc-52451
- Bu-1a/b Antibody (AV20) is a mouse monoclonal IgG1 Bu-1a/b antibody provided at 100 µg/ml
- raised against bursal cells from one day old H.B15 (Bu-1a/b) strain avians
- recommended for detection of Bu-1a and Bu-1b of avian origin by IP, IF and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Bu-1a/b Antibody (AV20). This work is in progress.
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Bu-1a/b Antibody (AV20) is a mouse monoclonal IgG1 antibody that detects Bu-1a/b protein of avian origin by immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Bu-1a/b (AV20) antibody is available in both non-conjugated and various conjugated forms, including fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. Bu-1 protein plays a crucial role in the regulation of cell death, which is vital for maintaining a functional immune system. During the development of T and B lymphocytes, Bu-1 protein is essential to eliminate nonfunctional or self-reactive cells to prevent autoimmune responses. Bu-1 antigens are genetically polymorphic type I transmembrane glycoproteins expressed on B cells and a subset of macrophages, indicating their importance in cell survival and adhesion during B cell maturation. Bu-1 presence in embryonic spleen and bone marrow cells marks these tissues as precursors for B cells, highlighting Bu-1 significance in early immune development. Bu-1 protein can trigger a rapid form of cell death akin to apoptosis, which is critical for the proper selection of functional lymphocytes. Bu-1a and Bu-1b variants represent the recessive and dominant allelic products of the Bu-1 gene, respectively, underscoring the genetic diversity within avian species.
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Bu-1a/b Antibody (AV20) References:
- Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens. | Veromaa, T., et al. 1988. Hybridoma. 7: 41-8. PMID: 2453446
- Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. | Houssaint, E., et al. 1989. Eur J Immunol. 19: 239-43. PMID: 2649381
- Genetic interaction between non-MHC T- and B-cell alloantigens in response to Rous sarcomas in chickens. | Gilmour, DG., et al. 1986. Immunogenetics. 23: 1-6. PMID: 3002975
- Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation. | Veromaa, T., et al. 1988. Eur J Immunol. 18: 225-30. PMID: 3258241
- Ontogeny and tissue distribution of the chicken Bu-1a antigen. | Houssaint, E., et al. 1987. Immunology. 62: 463-70. PMID: 3499381
- Influence of genotypes at a non-MHC B lymphocyte alloantigen locus (Bu-1) on expression of Ia (B-L) antigen on chicken bursal lymphocytes. | Fredericksen, TL. and Gilmour, DG. 1985. J Immunol. 134: 754-6. PMID: 3871215
- Committed precursors of B and T lymphocytes in chick embryo bursa of Fabricius, thymus, and bone marrow. | Brand, A., et al. 1983. Eur J Immunol. 13: 449-55. PMID: 6190659
- Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. | Rothwell, CJ., et al. 1996. Vet Immunol Immunopathol. 55: 225-34. PMID: 9014319
- The avian chB6 (Bu-1) alloantigen can mediate rapid cell death. | Funk, PE., et al. 1997. J Immunol. 159: 1695-702. PMID: 9257830
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bu-1a/b Antibody (AV20) | sc-52451 | 100 µg/ml | $322.00 | |||
Bu-1a/b Antibody (AV20) FITC | sc-52451 FITC | 100 tests in 2 ml | $336.00 |